Expression of miR-574 in placental tissue of preeclampsia and its significance in the pathogenesis of preeclampsia

YANG Ying; ZHU Yan-ju; GUO Yan-ling

doi:10.11659/jjssx.05E024042

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Expression of miR-574 in placental tissue of preeclampsia and its significance in the pathogenesis of preeclampsia

Basic Research | 更新时间：2025-04-17

- Expression of miR-574 in placental tissue of preeclampsia and its significance in the pathogenesis of preeclampsia
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 4, Pages: 300-304(2025)
- 作者机构：
  
  1.承德市妇幼保健院/承德市儿童医院产科，河北承德 067000
  2.承德医学院附属医院产科，河北承德 067000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E024042
  CLC： R714.244
- Received：09 May 2024，
  
  Published：25 April 2025
- 稿件说明：
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杨颖,朱艳菊,郭艳苓.子痫前期胎盘组织中miR-574的表达及其在子痫前期发病机制中的意义[J].局解手术学杂志,2025,34(04):300-304.

YANG Ying,ZHU Yan-ju,GUO Yan-ling.Expression of miR-574 in placental tissue of preeclampsia and its significance in the pathogenesis of preeclampsia[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(04):300-304.
杨颖,朱艳菊,郭艳苓.子痫前期胎盘组织中miR-574的表达及其在子痫前期发病机制中的意义[J].局解手术学杂志,2025,34(04):300-304. DOI： 10.11659/jjssx.05E024042.

YANG Ying,ZHU Yan-ju,GUO Yan-ling.Expression of miR-574 in placental tissue of preeclampsia and its significance in the pathogenesis of preeclampsia[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(04):300-304. DOI： 10.11659/jjssx.05E024042.

摘要

目的

探讨微小RNA-574（miR-574）在子痫前期（PE）孕妇胎盘组织中的表达及其在子痫前期发病机制中的意义。

方法

收集2021年3月至2023年6月承德市妇幼保健院产科收治的35例PE孕妇（PE组）和20例正常孕妇（正常组）的胎盘组织标本，采用qRT-PCR检测miR-574在不同胎盘组织中的表达。体外培养滋养细胞HTR8/Svneo，分为过表达组、抑制组、对照组，分别转染miR-574 mimics、miR-574 inhibitor、miR-NC。采用CCK-8实验检测各组细胞增殖能力；流式细胞术检测各组细胞凋亡率；Transwell小室实验检测各组细胞侵袭能力；Western blot检测各组细胞EMT相关蛋白表达。

结果

与正常组比较，PE组孕妇胎盘组织中miR-574表达明显升高（

0.05），并且重度PE孕妇的miR-574表达明显高于轻度PE孕妇（

0.05）。与对照组比较，过表达组细胞miR-574表达升高（

0.05），增殖能力下降（

0.05），凋亡率升高（

0.05），侵袭细胞数减少（

0.05），E-cadherin蛋白表达升高（

0.05），MMP9和N-cadherin蛋白表达降低（

0.05）。与对照组比较，抑制组细胞miR-574表达降低（

0.05），增殖能力升高（

0.05），凋亡率下降（

0.05），侵袭细胞数增多（

0.05），E-cadherin蛋白表达降低（

0.05），MMP9和N-cadherin蛋白表达升高（

0.05）。

结论

PE患者胎盘组织中miR-574表达增高，其能够通过影响滋养细胞增殖、凋亡、侵袭及EMT参与PE的发病过程。

Abstract

Objective

To investigate the expression of microRNA-574 （miR-574） in placental tissue of pregnant women with preeclampsia （PE） and its significance in the pathogenesis of preeclampsia.

Methods

The placental tissue samples of 35 PE pregnant women （the PE group） and 20 normal pregnant women （the normal group） admitted to the Department of Obstetrics in Chengde Maternal and Child Health Care Hospital from March 2021 to June 2023 were collected，and the expression of miR-574 in the different placental tissues was detected by qRT-PCR. The trophoblast cells HTR8/Svneo were cultured in vitro and divided into the overexpression group，inhibition group and control group，which were transfected with miR-574 mimics，miR-574 inhibitor and miR-NC，respectively. The cell proliferation ability of each group was detected by CCK-8 assay；The cell apoptosis rate of each group was detected by flow cytometry；The cell invasion ability of each group was detected by transwell chamber experiment；The expression of EMT related proteins of cells in each group was detected by western blot.

Results

Compared with the normal group，the miR-574 expression of placental tissue in the PE group was significantly increased （

0.05），and the expression of miR-574 in pregnant women with severe PE was significantly higher than that in pregnant women with mild PE （

0.05）. Compared with the control group，the expression of miR-574 of cells increased （

0.05），the cell proliferation ability decreased （

0.05），the cell apoptosis rate increased （

0.05），the number of invasive cells reduced （

0.05），the expression of E-cadherin protein increased （

0.05），and the expression of MMP9 and N-cadherin proteins decreased in the overexpression group （

0.05）. Compared with the control group，the expression of miR-574 of cells decreased （

0.05），the cell proliferation ability increased （

0.05），the cell apoptosis rate reduced （

0.05），the number of invasive cells increased （

0.05），the expression of E-cadherin protein decreased （

0.05），and the expression of MMP9 and N-cadherin proteins increased in the inhibition group （

0.05）.

Conclusion

The expression of miR-574 in the placental tissue of PE patients is increased，and it can participate in the pathogenesis of PE by influencing proliferation，apoptosis，invasion and EMT of trophoblast cell.

关键词

Keywords

references

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