Human umbilical cord mesenchymal stem cell culture supernatant promotes oral mucosal healing

ZHAO Qiang; YANG Ke; DOU Xing-fang; SUN You-han; CAI Yong-gang; YANG Wen-juan; GUO Feng-xia; ZHAO Hui; LIN Li

doi:10.11659/jjssx.04E025064

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Human umbilical cord mesenchymal stem cell culture supernatant promotes oral mucosal healing

Basic Research | 更新时间：2026-04-20

- Human umbilical cord mesenchymal stem cell culture supernatant promotes oral mucosal healing
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 4, Pages: 328-332(2026)
- 作者机构：
  
  甘肃省中心医院血液科，甘肃兰州 730050
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.04E025064
  CLC： R363.1+3;R781.5+1
- Received：11 April 2025，
  
  Published：25 April 2026
- 稿件说明：
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赵强,杨柯,豆兴芳等.人脐带间充质干细胞培养上清液促口腔黏膜愈合[J].局解手术学杂志,2026,35(04):328-332.

ZHAO Qiang,YANG Ke,DOU Xing-fang,et al.Human umbilical cord mesenchymal stem cell culture supernatant promotes oral mucosal healing[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(04):328-332.
赵强,杨柯,豆兴芳等.人脐带间充质干细胞培养上清液促口腔黏膜愈合[J].局解手术学杂志,2026,35(04):328-332. DOI： 10.11659/jjssx.04E025064.

ZHAO Qiang,YANG Ke,DOU Xing-fang,et al.Human umbilical cord mesenchymal stem cell culture supernatant promotes oral mucosal healing[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(04):328-332. DOI： 10.11659/jjssx.04E025064.

摘要

目的

探讨人脐带间充质干细胞（hUCMSCs）培养上清液对口腔黏膜愈合的促进作用。

方法

取1例足月生产健康产妇的新鲜脐带，分离hUCMSCs并培养，获取hUCMSCs培养上清液。取20只SD大鼠，在其口腔黏膜内局部注射顺铂注射液制备口腔黏膜炎模型，共18只造模成功。将造模成功的大鼠按照随机数字表法分为上清液治疗组（

=9）和对照组（

=9），分别予以hUCMSCs培养上清液、生理盐水治疗。观察干预前后溃疡数量、总溃疡面积和干预后溃疡愈合率，并采用HE染色观察2组残留溃疡组织病理改变。

结果

细胞形态学和分子表型流式细胞仪测试结果证实hUCMSCs分离成功。与干预前比较，干预后对照组溃疡数量、总溃疡面积变化无统计学差异（

0.05），上清液治疗组溃疡数量减少、总溃疡面积缩小（

0.05），且干预后上清液治疗组溃疡数量少于对照组、总溃疡面积小于对照组、溃疡愈合率高于对照组（

0.05）。上清液治疗组大鼠口腔黏膜细胞层次基本恢复、界限清晰，小血管数目多；对照组上皮全层丧失，符合顺铂导致的口腔黏膜炎病理改变。

结论

hUCMSCs培养上清液可促进化疗所致的口腔黏膜炎大鼠创面愈合，减轻病理改变。

Abstract

Objective

To explore the promoting effect of human umbilical cord mesenchymal stem cells （hUCMSCs） culture supernatant on oral mucosal healing.

Methods

Fresh umbilical cord tissue was obtained from one healthy full-term parturient. hUCMSCs were isolated and cultured， and then the culture supernatant of hUCMSCs was collected. Twenty SD rats were selected， and the model of oral mucositis was established by local injection of cisplatin into the oral mucosa. A total of 18 rats were successfully modeled.The rats with successful modeling were divided into the supernatant treatment group （

=9） and the control group （

=9） according to the random number table method， and were treated with hUCMSCs culture supernatant and normal saline， respectively. The number of ulcers， the total ulcer area before and after the intervention， and the ulcer healing rate after the intervention were observed. HE staining was used to observe the pathological changes of the residual ulcer tissues in the two groups.

Results

The results of flow cytometry tests on cell morphology and molecular phenotype confirmed the successful isolation of hUCMSCs. Compared with before intervention， the changes in the number of ulcers and total ulcer area in the control group after intervention showed no statistically significant difference （

0.05）. In the sup

ernatant treatment group， the number of ulcers decreased and the total ulcer area shrank （

0.05）. Moreover， after intervention， the number of ulcers in the supernatant treatment group was less than that in the control group， the total ulcer area was smaller than that in the control group， and the ulcer healing rate was higher than that in the control group （

0.05）. In the supernatant treatment group， the layers of rat oral mucosal cells were basically restored with clear boundaries and an increased number of small blood vessels. In the control group， the entire epithelium was lost， consistent with the pathological changes of cisplatin-induced oral mucositis.

Conclusion

The hUCMSCs culture supernatant can promote wound healing and alleviate pathological changes in rats with chemotherapy-induced oral mucositis.

关键词

Keywords

references

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