Study on the characteristics and mechanisms of skin damage in mice after high-voltage electric shock based on metabolomics

YANG Xiao; DENG Ping; CHEN Si-yu; LI Jing-dian; WANG Hui; YUE Yang; YU Zheng-ping; GAO Peng; PI Hui-feng

doi:10.11659/jjssx.04E024074

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Study on the characteristics and mechanisms of skin damage in mice after high-voltage electric shock based on metabolomics

Basic Research | 更新时间：2025-05-26

- Study on the characteristics and mechanisms of skin damage in mice after high-voltage electric shock based on metabolomics
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 5, Pages: 379-385(2025)
- 作者机构：
  
  陆军军医大学军事预防医学系军队劳动卫生学教研室/电磁辐射医学防护教育部重点实验室，重庆 400038
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.04E024074
  CLC： R135.99
- Received：16 April 2024，
  
  Published：25 May 2025
- 稿件说明：
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杨潇,邓平,陈思羽等.基于代谢组学研究高压电击后小鼠皮肤损伤的特点和机制[J].局解手术学杂志,2025,34(05):379-385.

YANG Xiao,DENG Ping,CHEN Si-yu,et al.Study on the characteristics and mechanisms of skin damage in mice after high-voltage electric shock based on metabolomics[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(05):379-385.
杨潇,邓平,陈思羽等.基于代谢组学研究高压电击后小鼠皮肤损伤的特点和机制[J].局解手术学杂志,2025,34(05):379-385. DOI： 10.11659/jjssx.04E024074.

YANG Xiao,DENG Ping,CHEN Si-yu,et al.Study on the characteristics and mechanisms of skin damage in mice after high-voltage electric shock based on metabolomics[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(05):379-385. DOI： 10.11659/jjssx.04E024074.

摘要

目的

基于代谢组学研究高压电击对皮肤的损伤效应，分析其造成的代谢差异，探究其致伤机理。

方法

将16只SPF级C57BL/6J雄性小鼠分为电击组（头部皮肤电击处理）和对照组（头部皮肤电击声光刺激），观察2组小鼠处理后皮肤外观情况。通过组织HE染色、EVG染色、Masson染色分析电击导致的组织病理学改变。采用GC-MS和LC-MS代谢组学分析电击暴露后皮肤代谢谱及组织代谢物改变情况并进行差异代谢物分析。合并所得的差异代谢物，并进行KEGG富集分析。

结果

高压电击后小鼠皮肤破损可至真皮层，表皮层部分增厚、掀起、分离，真皮层中皮肤附属器结构被破坏，大量炎症细胞浸润，肿胀明显，伴有充血，出现重度皮肤炎症反应，皮肤屏障功能受损。代谢组学分析提示，电击暴露后多出现代谢物改变。KEGG富集分析结果显示，电击显著影响癌症的中心碳代谢通路，戊糖磷酸路径，嘌呤代谢，甘氨酸、丝氨酸与苏氨酸代谢过程，氨基酸tRNA生物合成机制，甘油磷脂代谢路径，嘧啶代谢规律，糖酵解/糖异生，丙氨酸代谢过程，胰高血糖素信号通路等。

结论

高压电击可导致皮肤深度破损，扰乱其能量代谢、氨基酸代谢，严重干扰其抗氧化和DNA修复系统功能。

Abstract

Objective

To study the damage effect of high-voltage electric shock on skin based on metabolomics， analyze its metabolic differences， and explore its injury mechanism.

Methods

A total of 16 SPF C57BL/6J male mice were divided into the electric shock group （head skin received electric shock treatment） and control group （head skin received electric shock acoustic-optical stimulation）， and the skin appearance after treatment of mice in the two groups was observed. The histopathological changes caused by electric shock were analyzed by HE staining， EVG staining and Masson staining. GC-MS and LC-MS metabonomics were used to analyze the changes of skin metabolism spectrum and tissue metabolites after electric shock exposure， and the differential metabolites were analyzed. The obtained differential metabolites were combined and KEGG enrichment analysis was conducted.

Results

After high-voltage electric shock， the skin of mice could be damaged to the dermis， and the epidermis was partially thickened， lifted and separated. The structure of skin appendages in the dermis was destroyed， with a large number of inflammatory cells infiltrating and obvious swelling， accompanied by congestion， which led to severe skin inflammatory reaction and impaired skin barrier function. Metabonomics analysis suggested that the metabolites changed after electric shock exposure. KEGG enrichment analysis showed that electric shock significantly affected the central carbon metabolism pathway of cancer， pentose phosphate pathway， purine metabolism， glycine， serine and threonine metabolism processes， amino acid tRNA biosynthesis mechanism， glycerophospholipid metabolism pathway， pyrimidine metabolism pattern， glycolysis/gluconeogenesis， alanine metabolism process， glucagon signal pathway and so on.

Conclusion

High voltage electric shock can cause deep skin damage， disturb its energy metabolism and amino acid metabolism， and seriously interfere with its antioxidant and DNA repair system functions.

关键词

Keywords

references

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