Toxic effects of polystyrene microplastics on Caco-2 and IEC-6 cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 2, Pages: 120-126(2026)
- 作者机构：
陆军军医大学军事预防医学系军队劳动卫生学教研室/电磁辐射医学防护教育部重点实验室，重庆 400038
- 作者简介：
- 基金信息：
DOI：10.11659/jjssx.03E025147
CLC： R12Received：26 March 2025，
Published：25 February 2026
- 稿件说明：
移动端阅览
张文娟,陶嘉雯,马秦龙等.聚苯乙烯微塑料对Caco-2和IEC-6细胞的毒效应研究[J].局解手术学杂志,2026,35(02):120-126.
ZHANG Wen-juan,TAO Jia-wen,MA Qin-long,et al.Toxic effects of polystyrene microplastics on Caco-2 and IEC-6 cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(02):120-126.
张文娟,陶嘉雯,马秦龙等.聚苯乙烯微塑料对Caco-2和IEC-6细胞的毒效应研究[J].局解手术学杂志,2026,35(02):120-126. DOI： 10.11659/jjssx.03E025147.
ZHANG Wen-juan,TAO Jia-wen,MA Qin-long,et al.Toxic effects of polystyrene microplastics on Caco-2 and IEC-6 cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(02):120-126. DOI： 10.11659/jjssx.03E025147.
摘要
目的
2
观察聚苯乙烯微塑料（PS-MPs）对人结肠癌腺细胞Caco-2和大鼠小肠隐窝上皮细胞IEC-6的毒效应。
方法
2
培养Caco-2和IEC-6细胞后，荧光显微镜观察不同粒径（0.05 μm、0.50 μm）、不同浓度（0 μg/mL、10 μg/mL、20 μg/mL、40 μg/mL、80 μg/mL、160 μg/mL、320 μg/mL）的PS-MPs暴露24 h后进入细胞的情况。CCK-8法和TMA-DPH荧光膜探针检测不同粒径、不同浓度的PS-MPs暴露24 h、48 h、96 h对细胞活力和细胞膜流动性的影响。DCFH-DA荧光探针检测不同粒径、不同浓度PS-MPs暴露24 h、48 h对细胞内活性氧（ROS）水平的影响。
结果
2
0.05 μm、0.50 μm粒径的PS-MPs暴露24 h均可进入Caco-2和IEC-6细胞。0.05 μm、0.50 μm粒径的PS-MPs均可明显促进Caco-2细胞生长（
P
<
0.05），抑制IEC-6细胞生长（
P
<
0.05），显著增加Caco-2和IEC-6细胞中ROS水平（
P
<
0.05）。但实验所用粒径和浓度的PS-MPs均不影响Caco-2细胞和IEC-6细胞的细胞膜流动性。
结论
2
PS-MPs可以进入Caco-2和IEC-6细胞，通过影响细胞的活力，增加ROS水平，从而发挥其毒效应。
Abstract
Objective
2
To observe the toxic effects of polystyrene microplastics （PS-MPs） on human colon cancer adenocarcinoma cells Caco-2 and rat small intestinal crypt epithelial cells IEC-6.
Methods
2
Caco-2 and IEC-6 cells were cultured and exposed to PS-MPs of two sizes （0.05 μm and 0.50 μm） at various concentrations （0 μg/mL，10 μg/mL，20 μg/mL，40 μg/mL，80 μg/mL，160 μg/mL，320 μg/mL） for 24 hours， thereby observing cellular uptake using fluorescence microscopy. Cell viability was assessed by CCK-8 assay， and cell membrane fluidity was detected by the TMA-DPH fluorescent probe after different sizes and concentrations of PS-MPs exposure for 24 hours， 48 hours and 96 hours. Intracellular reactive oxygen species （ROS） levels were detected with the DCFH-DA fluorescent probe after different sizes and concentrations of PS-MPs exposure for 24 hours， 48 hours and 96 hours.
Results
2
Both 0.05 μm and 0.5 μm PS-MPs were internalized by Caco-2 and IEC-6 cells after exposure for 24 hours. Both sizes of PS-MPs significantly promoted the growth of Caco-2 cells （
P
<
0.05）， while inhibited the growth of IEC-6 cells （
P
<
0.05），and significantly increased intracellular ROS levels in Caco-2 and IEC-6 cells.However，the tested sizes and concentrations of PS-MPs showed no significant effect on the cell membrane fluidity of either Caco-2 or IEC-6 cells.
Conclusion
2
PS-MPs can be internalized by Caco-2 and IEC-6 cells， and exerts toxic effects by altering cell viability and increasing ROS levels.
关键词
Keywords
references
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