Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery

SU Kai; YU Qing-hua; CAO Jia-wang; LI Shou-zhuo; ZENG Chao

doi:10.11659/jjssx.03E024070

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Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery

Clinical Research | 更新时间：2025-01-21

- Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 2, Pages: 134-138(2025)
- 作者机构：
  
  1.中国人民解放军南部战区总医院心胸外科，广东广州 510030
  2.中国人民解放军陆军第七十四集团军医院神经内科，广东广州 510220
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.03E024070
  CLC： R734.2
- Published：25 February 2025，
  
  Received：12 March 2024，
- 稿件说明：
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SU KAI, YU QING-HUA, CAO JIA-WANG, et al. Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery. [J]. Journal of regional anatomy and operative surgery, 2025, 34(2): 134-138.
DOI：

SU KAI, YU QING-HUA, CAO JIA-WANG, et al. Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery. [J]. Journal of regional anatomy and operative surgery, 2025, 34(2): 134-138. DOI： 10.11659/jjssx.03E024070.

摘要

目的

探讨非小细胞肺癌（NSCLC）患者血清长链非编码RNA X无活性特异性转录物（lncRNA XIST）、微小RNA-126-3p（miR-126-3p）表达及其与根治术后复发的关系。

方法

选取2019年2月至2020年10月中国人民解放军南部战区总医院收治的108例行根治术的NSCLC患者作为NSCLC组，选取同期在该院体检的健康志愿者52例作为对照组。采用qRT-PCR法检测血清lncRNA XIST、miR-126-3p相对表达水平；

Pearson

法分析血清lncRNA XIST与miR-126-3p表达的相关性；

Logistic

回归分析NSCLC患者术后复发的影响因素；受试者工作特征（

ROC

）曲线评估血清lncRNA XIST、miR-126-3p对患者术后复发的预测价值。

结果

NSCLC组患者血清lncRNA XIST表达水平高于对照组（

0.05），miR-126-3p表达水平则低于对照组（

0.05）。血清lncRNA XIST、miR-126-3p表达水平与TNM分期、淋巴结转移有关（

0.05）。生物信息学网站预测显示，lncRNA XIST与miR-126-3p存在靶向结合位点，且血清lncRNA XIST与miR-126-3p表达呈负相关（

=-0.579，

0.05）。与未复发组相比，复发组患者血清lncRNA XIST表达水平升高（

0.05），miR-126-3p表达水平降低（

0.05）。

Logistic

回归分析结果显示，lncRNA XIST、miR-126-3p、淋巴结转移、TNM分期是NSCLC患者术后复发的影响因素（

0.05）；血清lncRNA XIST、miR-126-3p及二者联合预测NSCLC患者术后复发的曲线下面积（

AUC

）分别为0.750、0.886、0.933，二者联合预测优于单独预测（

二者联合

vs.

lncRNA XIST

=4.076、

二者联合

vs.

miR-126-3p

=2.065，

0.05）。

结论

NSCLC患者血清lncRNA XIST表达升高，miR-126-3p表达降低，两者对于患者根治术后复发具有一定的预测价值。

Abstract

Objective

To investigate the expression of serum long non-coding RNA X-inactive specific transcript （lncRNA XIST） and microRNA-126-3p （miR-126-3p） in patients with non-small cell lung cancer （NSCLC），and their relationship with recurrence after radical surgery.

Methods

A total of 108 NSCLC patients who underwent radical surgery and were admitted to General Hospital of Southern Theater Command of the People's Liberation Army of China from February 2019 to October 2020 were selected as the NSCLC group，and 52 healthy volunteers who underwent physical examination in this hospital were selected as the control group. qRT-PCR method was used to detect the relative expression levels of serum lncRNA XIST and miR-126-3p.

Pearson

method was used to analyze the correlation between serum lncRNA XIST and miR-126-3p expression.

Logistic

regression analysis was used to analyze the influencing factors of postoperative recurrence in NSCLC patients.

Receiver operating characteristic

（

ROC

） curve was used to evaluate the predictive value of serum lncRNA XIST and miR-126-3p on postoperative recurrence in patients.

Results

The expression level of serum lncRNA XIS

T in the NSCLC group was higher than that in the control group （

0.05），while the expression level of miR-126-3p was lower than that in the control group （

0.05）. The expression levels of serum lncRNA XIST and miR-126-3p were related to TNM staging and lymph node metastasis （

0.05）. Bioinformatics website predicted that there was a targeted binding site between lncRNA XIST and miR-126-3p，and serum lncRNA XIST was negatively correlated with miR-126-3p expression （

=-0.579，

0.05）. Compared with the non-recurrent group，the expression level of serum lncRNA XIST in the recurrent group increased （

0.05），while the expression level of miR-126-3p in the recurrent group decreased （

0.05）.

Logistic

regression analysis results showed that lncRNA XIST，miR-126-3p，lymph node metastasis，and TNM staging were the influencing factors for postoperative recurrence in NSCLC patients （

0.05）. The

area under the curve

（

AUC

） of serum lncRNA XIST，miR-126-3p，and the combination of the two in prediction of postoperative recurrence in NSCLC patients were 0.750，0.886，and 0.933，respectively，the combined prediction of the two was superior to individual prediction （

combination

. lncRNA XIST

=4.076，

combination

.miR-126-3p

=2.065，

0.05）.

Conclusion

The expression of serum lncRNA XIST is increased and miR-126-3p is decreased in NSCLC patients，both of which have certain predictive value for recurrence after radical surgery in patients.

关键词

非小细胞肺癌lncRNA XISTmiRNA-126-3p根治术复发

Keywords

non-small cell lung cancerlncRNA XISTmiRNA-126-3pradical surgeryrecurrence

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