Exploring key pathways in psoriasis development via RNA sequencing of mouse skin

LONG Shuang; WANG Guo-jian; ZHAO Na; WANG Tao

doi:10.11659/jjssx.02E026003

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Exploring key pathways in psoriasis development via RNA sequencing of mouse skin

Basic Research | 更新时间：2026-07-24

- Exploring key pathways in psoriasis development via RNA sequencing of mouse skin
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 7, Pages: 602-609(2026)
- 作者机构：
  
  陆军军医大学军事预防医学系防原医学教研室/全军复合伤研究所/重庆市纳米医学工程研究中心，重庆 400038
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.02E026003
  CLC： R758.63
- Received：01 February 2026，
  
  Online First：24 July 2026，
  
  Published：25 July 2026
- 稿件说明：
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龙爽,汪国建,赵娜等.基于小鼠皮肤转录组测序探索银屑病发生发展的关键通路[J].局解手术学杂志,2026,35(07):602-609.

LONG Shuang,WANG Guo-jian,ZHAO Na,et al.Exploring key pathways in psoriasis development via RNA sequencing of mouse skin[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(07):602-609.
龙爽,汪国建,赵娜等.基于小鼠皮肤转录组测序探索银屑病发生发展的关键通路[J].局解手术学杂志,2026,35(07):602-609. DOI： 10.11659/jjssx.02E026003.

LONG Shuang,WANG Guo-jian,ZHAO Na,et al.Exploring key pathways in psoriasis development via RNA sequencing of mouse skin[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(07):602-609. DOI： 10.11659/jjssx.02E026003.

摘要

目的

运用皮肤高通量测序结合生物信息学分析筛选小鼠银屑病发展的关键通路。

方法

小鼠头颈后部皮肤局部涂抹咪喹莫特（IMQ），建立小鼠银屑病模型；对银屑病模型小鼠和对照小鼠皮肤进行转录组测序，结合生物信息学分析差异表达基因（DEGs），筛选关键通路；RT-PCR验证部分关键通路基因的表达差异。

结果

银屑病组小鼠皮损处表皮厚度增加，红斑、浸润、鳞屑评分及总评分均增高，提示小鼠银屑病模型构建成功。转录组测序共筛选出4 752个DEGs，其中2 276个显著上调，2 476个显著下调；上调DEGs主要富集于细胞因子-细胞因子受体互作、中性粒细胞外诱捕网形成、NF-κB及IL-17信号通路等炎症相关通路，下调DEGs主要富集于过氧化物酶体、AMPK信号通路、PPAR信号通路以及各种脂质代谢通路等。进一步采用RT-PCR验证部分基因表达发现，与对照组相比，银屑病组小鼠皮肤组织中脂质代谢相关分子ELOVL4、SCD1、FAR2、FA2H、GSTM1 mRNA的表达下调， CXCL1、CXCL2、CXCL3、IL-1β、TNF-α mRNA表达上调，差异均具有统计学意义（

0.05）。

结论

银屑病小鼠皮肤呈现免疫炎症过度激活、脂质与能量代谢广泛抑制的特征，炎症与脂质代谢通路形成相互作用的恶性循环；脂质代谢相关通路及关键基因可作为银屑病潜在的防治靶点。

Abstract

Objective

To identify the key pathways in the development of psoriasis in mice by using high-throughput sequencing of skin combined with bioinformatics analysis.

Methods

Imiquimod （IMQ） was applied locally to the skin at the back of the head and neck of mice to establish a mouse model of psoriasis. RNA sequencing was performed on the skin of the psoriasis model mice and control mice， followed by bioinformatics analysis of differentially expressed genes （DEGs） to screen out key pathways. The expression differences of some key pathway genes were verified by RT-PCR.

Results

In the psoriasis group of mice， the epidermal thickness at the lesion site increased， and the scores of erythema， infiltration， and scales， as well as the total score all increased， sugges

ting the successful establishment of the mouse psoriasis model. RNA sequencing screened out a total of 4 752 DEGs， among which 2 276 were significantly up-regulated and 2 476 were significantly down-regulated. The upregulated DEGs were predominantly enriched in inflammation-related pathways such as cytokine-cytokine receptor interaction， neutrophil extracellular trap formation， NF-κB and IL-17 signaling pathways， etc. The down-regulated DEGs were predominantly enriched in peroxisome， AMPK signaling pathway， PPAR signaling pathway， and various lipid metabolism pathways， etc.Further verification of the expression of some genes by RT-PCR revealed that， compared with the control group， the mRNA expressions of lipid metabolism-related molecules such as ELOVL4， SCD1， FAR2， FA2H， and GSTM1 in the skin tissues of mice in the psoriasis group were down-regulated，the mRNA expressions of CXCL1， CXCL2， CXCL3， IL-1β and TNF-α were up-regulated， and the differences were statistically significant （

0.05）.

Conclusion

The skin of psoriatic mice is characterized by excessive activation of immune inflammation and widespread suppression of lipid and energy metabolism. with a vicious cycle of interplay between inflammatory and lipid metabolism pathways. The pathways and key genes related to lipid metabolism can serve as potential therapeutic targets for psoriasis.

关键词

Keywords

references

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