Application value of metagenomic next-generation sequencing technology in the diagnosis of spinal infections

TU Bing; CHEN Hu; CHEN Jun-lin; MA Xiang-yang; YI Hong-lei; CAI Xue-jiu

doi:10.11659/jjssx.01E025123

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Application value of metagenomic next-generation sequencing technology in the diagnosis of spinal infections

Clinical Research | 更新时间：2026-01-19

- Application value of metagenomic next-generation sequencing technology in the diagnosis of spinal infections
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 1, Pages: 28-31(2026)
- 作者机构：
  
  中国人民解放军南部战区总医院脊柱外科，广东广州 510010
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.01E025123
  CLC： R681.5;R446.5
- Received：23 January 2025，
  
  Published：25 January 2026
- 稿件说明：
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涂兵,陈虎,陈钧麟等.宏基因组二代测序技术在脊柱感染诊断中的应用[J].局解手术学杂志,2026,35(01):28-31.

TU Bing,CHEN Hu,CHEN Jun-lin,et al.Application value of metagenomic next-generation sequencing technology in the diagnosis of spinal infections[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(01):28-31.
涂兵,陈虎,陈钧麟等.宏基因组二代测序技术在脊柱感染诊断中的应用[J].局解手术学杂志,2026,35(01):28-31. DOI： 10.11659/jjssx.01E025123.

TU Bing,CHEN Hu,CHEN Jun-lin,et al.Application value of metagenomic next-generation sequencing technology in the diagnosis of spinal infections[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(01):28-31. DOI： 10.11659/jjssx.01E025123.

摘要

目的

探讨宏基因组二代测序（mNGS）技术在脊柱感染诊断中的应用价值。

方法

回顾性分析2021年1月至2024年10月我院脊柱外科收治的30例疑似脊柱感染患者的临床资料。经手术或CT引导下穿刺获取样本，分别进行mNGS检测、细菌培养及组织病理学检查。以组织病理学结果为金标准，将患者分为感染组和未感染组，比较2组患者的一般资料及感染指标，分析mNGS和细菌培养诊断脊柱感染的特异性、敏感性及检测时间。

结果

组织病理学结果显示，4例为无菌性炎症，26例确诊为脊柱感染。2组患者在年龄、性别及红细胞沉降率（ESR）、C反应蛋白（CRP）、白细胞计数（WBC）等指标方面差异均无统计学意义（

0.05）。在26例脊柱感染患者中，mNGS检测阳性23例，细菌培养阳性9例。mNGS可检出多种病原微生物，包括金黄色葡萄球菌、大肠埃希菌、结核分枝杆菌及厌氧菌等，且部分病例在细菌培养阴性时仍能通过mNGS明确病原学诊断。mNGS检测时间明显短于细菌培养（

0.05）。mNGS诊断脊柱感染的敏感性明显高于细菌培养（

0.05），但两种方法诊断的特异性比较差异无统计学意义（

0.05）。

结论

mNGS在脊柱感染的病原学诊断中具有更快的检测速度和更高的敏感性，可有效弥补传统细菌培养的局限，有望在疑似脊柱感染患者的病原学诊断中广泛应用。

Abstract

Objective

To explore the application value of metagenomic next-generation sequencing （mNGS） technology in the diagnosis of spinal infections.

Methods

A retrospective analysis was conducted on 30 patients with suspected spinal infections who were treated in the department of spine surgery of our hospital from January 2021 to October 2024. Samples obtained by surgery or CT-guided puncture were subjected to mNGS detection， bacterial culture and histopathological examination respectively. Taking histopathological results as the gold standard，the patients were divided into the infected group and the uninfected group. The general data and infection indicators of patients in the two groups were compared， and the specificity， sensitivity and turnaround time of mNGS and bacterial culture in diagnosing spinal infection were analyzed.

Results

Histopathological results showed that 4 cases were aseptic inflammation and 26 cases were diagnosed as spinal infection. There were no statistically significant differences in the age， gender， erythrocyte sedimentation rate （ESR）， C-reactive protein （CRP），or white blood cell count （WBC） of patients between the two groups （

0.05）. Among the 26 patients with spinal infections， 23 cases were positive for mNGS detection and 9 cases were positive for bacterial culture. mNGS could detect a variety of pathogenic microorganisms， including Staphylococcus aureus， Escherichia coli， Mycobacterium tuberculosis and anaerobic bacteria， etc. Furthermore， mNGS established an etiological diagnosis in some cases where bacterial culture was negative.The turnaround time of mNGS was significantly shorter than that of bacterial culture（

0.05）.The sensitivity of mNGS in diagnosing spinal infections was significantly higher than that of bacterial culture （

0.05）， but there was no statistically significant difference in the specificity of the two diagnostic methods （

0.05）.

Conclusion

mNGS has a faster turnaround time and higher sensitivity in the etiological diagnosis of spinal infections， which can effectively make up for the limitations of traditional bacterial culture and is expected to be widely used in the etiological diagnosis of patients suspected of spinal infections.

关键词

Keywords

references

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