Effect of a novel resuscitation fluid on coagulation dysfunction in septic rats

WU Fang-qi; FANG Hui; XIONG Jiong; LI Ting-ting; DENG Fu-yu; TANG Song-xun; LIU Xu; TANG Yan

doi:10.11659/jjssx.01E025116

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Effect of a novel resuscitation fluid on coagulation dysfunction in septic rats

Basic Research | 更新时间：2026-01-19

- Effect of a novel resuscitation fluid on coagulation dysfunction in septic rats
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 1, Pages: 23-27(2026)
- 作者机构：
  
  1.贵州医科大学临床医学院，贵州贵阳 550004
  2.贵州医科大学附属医院重症医学科，贵州贵阳 550004
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.01E025116
  CLC： R631+.2
- Received：22 January 2025，
  
  Published：25 January 2026
- 稿件说明：
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吴方琪,方慧,熊炯等.一种新型复苏液对脓毒症大鼠凝血功能障碍的影响研究[J].局解手术学杂志,2026,35(01):23-27.

WU Fang-qi,FANG Hui,XIONG Jiong,et al.Effect of a novel resuscitation fluid on coagulation dysfunction in septic rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(01):23-27.
吴方琪,方慧,熊炯等.一种新型复苏液对脓毒症大鼠凝血功能障碍的影响研究[J].局解手术学杂志,2026,35(01):23-27. DOI： 10.11659/jjssx.01E025116.

WU Fang-qi,FANG Hui,XIONG Jiong,et al.Effect of a novel resuscitation fluid on coagulation dysfunction in septic rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(01):23-27. DOI： 10.11659/jjssx.01E025116.

摘要

目的

探究一种由腺苷、利多卡因及硫酸镁（ALM）组成的新型复苏液对脓毒症大鼠凝血功能障碍的影响。

方法

将48只雄性SD大鼠随机分成ALM治疗组、脓毒症组和假手术组，每组16只。假手术组大鼠在开腹检查盲肠后直接缝合；ALM治疗组和脓毒症组大鼠均通过盲肠结扎穿刺建立脓毒症模型。造模后2 h，ALM治疗组大鼠通过尾静脉注入0.3 mL的ALM复苏液，随后以0.5 mL·kg

-1

·h

-1

的速度持续输注4 h；脓毒症组和假手术组大鼠在相同时间注入等量的0.9%氯化钠注射液。测定各组大鼠造模后6、12 h血浆凝血功能指标凝血酶原时间（PT）、活化部分凝血酶原时间（APTT），组织因子（TF）及血管内皮损伤指标内皮多糖包被核心蛋白Syndecan-1、细胞间黏附分子-1（ICAM-1）的浓度。

结果

与假手术组比较，ALM治疗组及脓毒症组大鼠造模后12 h的PT延长，造模后6 h和12 h的APTT及TF、ICAM-1、Syndecan-1水平延长/升高（

0.01)。与脓毒血症组比较，ALM治疗组大鼠造模后12 h的PT显著缩短，造模后6 h和12 h的APTT及TF、ICAM-1、Syndecan-1水平显著缩短/降低（

0.01)。

结论

ALM复苏液能够减少内皮多糖包被的降解，抑制血管内皮细胞与炎症细胞黏附，减少TF释放，从而改善脓毒症大鼠的凝血功能。

Abstract

Objective

To explore the impact of a novel resuscitation fluid comprising adenosine， lidocaine and magnesium（ALM）on the coagulation dysfunction in septic rats.

Methods

A total of 48 male Sprague-Dawley rats were randomly assigned to the ALM treatment group， sepsis group，and sham surgery group，with 16 rats in each group. Rats in the sham surgery group had their cecum directly sutured after laparotomy examination， and rats both in the ALM treatment group and the sepsis group were established septic models by cecal ligation and puncture. Two hours after modeling，rats in the ALM treatment group were administrated 0.3 mL of ALM resuscitation fluid via tail vein injection，followed by continuous infusion of ALM resuscitation fluid at a rate of 0.5 mL·kg

-1

·h

-1

； rats in the sepsis group and the sham surgery group were injected with the same amount of 0.9% sodium chloride solution at the same time. The prothrombin time （PT）， activated partial thromboplastin time （APTT）， and concentrations of tissue factor （TF），endothelial injury marker of endothelial glycocalyx core protein Syndecan-1，and intercellular adhesion molecule-1（ICAM-1） of rats in each grou

p were detected.

Results

Compared with the sham surgery group， the PT 12 hours after modeling of rats in the ALM treatment group and the sepsis group prolonged， and the APTT as well as the levels of TF， ICAM-1， and Syndecan-1 6 hours and 12 hours after modeling prolonged/elevated （

0.01）.Compared with the sepsis group， the PT 12 hours after modeling of rats in the ALM treatment group significantly shortened， and the APTT as well as levels of TF， ICAM-1， and Syndecan-1 6 hours and 12 hours after modeling significantly shortened/reduced（

0.01）.

Conclusion

ALM resuscitation fluid attenuates coagulation dysfunction in septic rats through reducing the degradation of endothelial glycocalyx，inhibiting the adhesion between endothelial cells and inflammatory cells，as well as decreasing the release of TF.

关键词

Keywords

references

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