Development of 3D printed electrospun vascular graft loaded with tetramethylpyrazine for reducing thrombosis and restraining aneurysmal dilatation

Yihong Shen; Yanjun Pan; Fubang Liang; Jiahui Song; Xiao Yu; Jie Cui; Guangfang Cai; Mohamed EL-Newehy; Meera Moydeen Abdulhameed; Hongbing Gu; Binbin Sun; Meng Yin; Xiumei Mo

doi:10.1093/burnst/tkae008

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Development of 3D printed electrospun vascular graft loaded with tetramethylpyrazine for reducing thrombosis and restraining aneurysmal dilatation

更新时间：2024-04-19

- Development of 3D printed electrospun vascular graft loaded with tetramethylpyrazine for reducing thrombosis and restraining aneurysmal dilatation
- Burns & Trauma Vol. 12, Issue 1, (2024)
- 作者机构：
  
  1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials
  2. Donghua University
  3. Department of Cardiothoracic Surgery
  4. Shanghai Jiao Tong University
  5. Department of Chemistry
  6. King Saud University
  7. Department of Cardiovascular Surgery
  8. Shanghai Jiao Tong University School of Medicine
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkae008
  CLC：
- Published：2024，
  
  Published Online：8 April 2024，
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Yihong Shen, Yanjun Pan, Fubang Liang, Jiahui Song, Xiao Yu, Jie Cui, Guangfang Cai, Mohamed EL-Newehy, Meera Moydeen Abdulhameed, Hongbing Gu, Binbin Sun, Meng Yin, Xiumei Mo, Development of 3D printed electrospun vascular graft loaded with tetramethylpyrazine for reducing thrombosis and restraining aneurysmal dilatation, Burns & Trauma, Volume 12, 2024, tkae008, https://doi.org/10.1093/burnst/tkae008
DOI：

Yihong Shen, Yanjun Pan, Fubang Liang, Jiahui Song, Xiao Yu, Jie Cui, Guangfang Cai, Mohamed EL-Newehy, Meera Moydeen Abdulhameed, Hongbing Gu, Binbin Sun, Meng Yin, Xiumei Mo, Development of 3D printed electrospun vascular graft loaded with tetramethylpyrazine for reducing thrombosis and restraining aneurysmal dilatation, Burns & Trauma, Volume 12, 2024, tkae008, https://doi.org/10.1093/burnst/tkae008 DOI：

摘要

Abstract

BackgroundSmall-diameter vascular grafts have become the focus of attention in tissue engineering. Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery. Therefore

we focused on fabricating 3D printed electrospun vascular grafts loaded with tetramethylpyrazine (TMP) to overcome these limitations.MethodsBased on electrospinning and 3D printing

3D-printed electrospun vascular grafts loaded with TMP were fabricated. The inner layer of the graft was composed of electrospun poly(L-lactic-co-caprolactone) (PLCL) nanofibers and the outer layer consisted of 3D printed polycaprolactone (PCL) microfibers. The characterization and mechanical properties were tested. The blood compatibility and in vitro cytocompatibility of the grafts were also evaluated. Additionally

rat abdominal aortas were replaced with these 3D-printed electrospun grafts to evaluate their biosafety.ResultsMechanical tests demonstrated that the addition of PCL microfibers could improve the mechanical properties. In vitro experimental data proved that the introduction of TMP effectively inhibited platelet adhesion. Afterwards

rat abdominal aorta was replaced with 3D-printed electrospun grafts. The 3D-printed electrospun graft loaded with TMP showed good biocompatibility and mechanical strength within 6 months and maintained substantial patency without the occurrence of acute thrombosis. Moreover

no obvious aneurysmal dilatation was observed.ConclusionsThe study demonstrated that 3D-printed electrospun vascular grafts loaded with TMP may have the potential for injured vascular healing.

关键词

Keywords

3D printingElectrospinningTetramethylpyrazineThrombosisAneurysmal dilatationVascular graft

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