Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion

Jing Du; Wei Liu; Yajuan Song; Yu Zhang; Chen Dong; Shaoheng Xiong; Zhaosong Huang; Tong Wang; Jianke Ding; Qiang He; Zhou Yu; Xianjie Ma

doi:10.1093/burnst/tkad057

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Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion

更新时间：2024-04-19

- Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion
- Burns & Trauma Vol. 12, Issue 1, (2024)
- 作者机构：
  
  1. Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University
  2. Key Laboratory of Aerospace Medicine of Ministry of Education
  3. Fourth Military Medical University
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkad057
  CLC：
- Published：2024，
  
  Published Online：7 February 2024，
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Jing Du, Wei Liu, Yajuan Song, Yu Zhang, Chen Dong, Shaoheng Xiong, Zhaosong Huang, Tong Wang, Jianke Ding, Qiang He, Zhou Yu, Xianjie Ma, Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion, Burns & Trauma, Volume 12, 2024, tkad057, https://doi.org/10.1093/burnst/tkad057
DOI：

Jing Du, Wei Liu, Yajuan Song, Yu Zhang, Chen Dong, Shaoheng Xiong, Zhaosong Huang, Tong Wang, Jianke Ding, Qiang He, Zhou Yu, Xianjie Ma, Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion, Burns & Trauma, Volume 12, 2024, tkad057, https://doi.org/10.1093/burnst/tkad057 DOI：

摘要

Abstract

BackgroundTissue expansion

a technique in which skin regeneration is induced by mechanical stretch stimuli

is commonly used for tissue repair and reconstruction. In this study

we aimed to monitor the autophagy levels of expanded skin after the application of expansion stimuli and explore the effect of autophagy modulation on skin regeneration.MethodsA rat scalp expansion model was established to provide a stable expanded skin response to mechanical stretch. Autophagy levels at different time points (6

48 and 72 h after the last expansion) were detected via western blotting. The effect of autophagy regulation on skin regeneration during tissue expansion was evaluated via skin expansion efficiency assessment

western blotting

immunofluorescence staining

TUNEL staining and laser Doppler blood flow imaging.ResultsThe autophagic flux reached its highest level 48 h after tissue expansion. Activating autophagy by rapamycin increased the area of expanded skin as well as the thicknesses of epidermis and dermis. Furthermore

activating autophagy accelerated skin regeneration during tissue expansion by enhancing the proliferation of cells and the number of epidermal basal and hair follicle stem cells

reducing apoptosis

improving angiogenesis

and promoting collagen synthesis and growth factor secretion. Conversely

the regenerative effects were reversed when autophagy was blocked.ConclusionsAutophagy modulation may be a promising therapeutic strategy for improving the efficiency of tissue expansion and preventing the incidence of the complication of skin necrosis.

关键词

Keywords

AutophagyMechanical stretchSkin regenerationTissue expansion

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