Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats
- Burns & Trauma Vol. 11, Issue 1, (2023)
- 作者机构：
1. Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University
2. The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi medical University
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkad013
CLC：Published：2023，
Published Online：27 April 2023，
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Taotao Hu, Shusen Chang, Fang Qi, Zhonghui Zhang, Jiayin Chen, Lingli Jiang, Dali Wang, Chengliang Deng, Kaiyu Nie, Guangchao Xu, Zairong Wei, Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats, Burns & Trauma, Volume 11, 2023, tkad013, https://doi.org/10.1093/burnst/tkad013
Taotao Hu, Shusen Chang, Fang Qi, Zhonghui Zhang, Jiayin Chen, Lingli Jiang, Dali Wang, Chengliang Deng, Kaiyu Nie, Guangchao Xu, Zairong Wei, Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats, Burns & Trauma, Volume 11, 2023, tkad013, https://doi.org/10.1093/burnst/tkad013 DOI：
摘要
Abstract
BackgroundSchwann cell-like cells (SCLCs)
differentiated from mesenchymal stem cells
have shown promising outcomes in the treatment of peripheral nerve injuries in preclinical studies. However
certain clinical obstacles limit their application. Hence
the primary aim of this study was to investigate the role of exosomes derived from SCLCs (SCLCs-exo) in peripheral nerve regeneration.MethodsSCLCs were differentiated from human amniotic mesenchymal stem cells (hAMSCs) in vitro and validated by immunofluorescence
real-time quantitative PCR and western blot analysis. Exosomes derived from hAMSCs (hAMSCs-exo) and SCLCs were isolated by ultracentrifugation and validated by nanoparticle tracking analysis
WB analysis and electron microscopy. A prefabricated nerve graft was used to deliver hAMSCs-exo or SCLCs-exo in an injured sciatic nerve rat model. The effects of hAMSCs-exo or SCLCs-exo on rat peripheral nerve injury (PNI) regeneration were determined based on the recovery of neurological function and histomorphometric variation. The effects of hAMSCs-exo or SCLCs-exo on Schwann cells were also determined via cell proliferation and migration assessment.ResultsSCLCs significantly expressed the Schwann cell markers glial fibrillary acidic protein and S100. Compared to hAMSCs-exo
SCLCs-exo significantly enhanced motor function recovery
attenuated gastrocnemius muscle atrophy and facilitated axonal regrowth
myelin formation and angiogenesis in the rat model. Furthermore
hAMSCs-exo and SCLCs-exo were efficiently absorbed by Schwann cells. However
compared to hAMSCs-exo
SCLCs-exo significantly promoted the proliferation and migration of Schwann cells. SCLCs-exo also significantly upregulated the expression of a glial cell-derived neurotrophic factor
myelin positive regulators (SRY-box transcription factor 10
early growth response protein 2 and organic cation/carnitine transporter 6) and myelin proteins (myelin basic protein and myelin protein zero) in Schwann cells.ConclusionsThese findings suggest that SCLCs-exo can more efficiently promote PNI regeneration than hAMSCs-exo and are a potentially novel therapeutic approach for treating PNI.
关键词
Keywords
Schwann cell-like cellsExosomesPeripheral nerve injuryMesenchymal stem cellsNerve regeneration
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