CD9 negatively regulates collective electrotaxis of the epidermal monolayer by controlling and coordinating the polarization of leader cells
- Burns & Trauma Vol. 11, Issue 1, (2023)
- 作者机构：
1. Department of Plastic Surgery
2. Third Military Medical University (Army Medical University)
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkad012
CLC：Published：2023，
Published Online：24 July 2023，
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Xiaoqiang Liu, Jinrui Yang, Meng Kong, Min Jiang, Luojia Liu, Jinghong Zhang, Ying Chen, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jie Liu, Jiaping Zhang, CD9 negatively regulates collective electrotaxis of the epidermal monolayer by controlling and coordinating the polarization of leader cells, Burns & Trauma, Volume 11, 2023, tkad012, https://doi.org/10.1093/burnst/tkad012
Xiaoqiang Liu, Jinrui Yang, Meng Kong, Min Jiang, Luojia Liu, Jinghong Zhang, Ying Chen, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jie Liu, Jiaping Zhang, CD9 negatively regulates collective electrotaxis of the epidermal monolayer by controlling and coordinating the polarization of leader cells, Burns & Trauma, Volume 11, 2023, tkad012, https://doi.org/10.1093/burnst/tkad012 DOI：
摘要
Abstract
BackgroundEndogenous electric fields (EFs) play an essential role in guiding the coordinated collective migration of epidermal cells to the wound centre during wound healing. Although polarization of leadercells is essential for collective migration
the signal mechanisms responsible for the EF-induced polarization of leader cells under electrotactic collective migration remain unclear. This study aims to determine how the leader cells are polarized and coordinated during EF-guided collective migration of epidermal cell sheets.MethodsCollective migration of the human epidermal monolayer (human immortalized keratinocytes HaCaT) under EFs was observed via time-lapse microscopy. The involvement of tetraspanin-29 (CD9) in EF-induced fibrous actin (F-actin) polarization of leader cells as well as electrotactic migration of the epidermal monolayer was evaluated by genetic manipulation. Blocking
rescue and co-culture experiments were conducted to explore the downstream signalling of CD9.ResultsEFs guided the coordinated collective migration of the epithelial monolayer to the anode
with dynamic formation of pseudopodia in leader cells at the front edge of the monolayer along the direction of migration. F-actin polarization
as expected
played an essential role in pseudopod formation in leader cells under EFs. By confocal microscopy
we found that CD9 was colocalized with F-actin on the cell surface and was particularly downregulated in leader cells by EFs. Interestingly
genetic overexpression of CD9 abolished EF-induced F-actin polarization in leader cells as well as collective migration in the epidermal monolayer. Mechanistically
CD9 determined the polarization of F-actin in leader cells by downregulating a disintegrin and metalloprotease 17/heparin-binding epidermal growth factor-like growth factor/epidermal growth factor receptor (ADAM17/HB-EGF/EGFR) signalling. The abolished polarization of leader cells due to CD9 overexpression could be restored in a co-culture monolayer where normal cells and CD9-overexpressing cells were mixed; however
this restoration was eliminated again by the addition of the HB-EGF-neutralizing antibody.ConclusionCD9 functions as a key regulator in the EF-guided collective migration of the epidermal monolayer by controlling and coordinating the polarization of leader cells through ADAM17/HB-EGF/EGFR signalling.
关键词
Keywords
CD9Electric fieldsCollective migrationWound healingF-actin polarizationleader cells
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