Prospective study and validation of early warning marker discovery based on integrating multi-omics analysis in severe burn patients with sepsis

Jiamin Huang; Yi Chen; Zaiwen Guo; Yanzhen Yu; Yi Zhang; Pingsong Li; Lei Shi; Guozhong Lv; Bingwei Sun

doi:10.1093/burnst/tkac050

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Prospective study and validation of early warning marker discovery based on integrating multi-omics analysis in severe burn patients with sepsis

更新时间：2023-05-16

- Prospective study and validation of early warning marker discovery based on integrating multi-omics analysis in severe burn patients with sepsis
- Burns & Trauma Vol. 11, Issue 1, (2023)
- 作者机构：
  
  1. Department of Burns and Plastic Surgery
  2. Medical University
  3. Department of Burns and Plastic Surgery, Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University
  4. Department of Burns and Plastic Surgery, Affiliated Hospital of Nantong University
  5. Department of Burns and Plastic Surgery, Northern Jiangsu People’s Hospital
  6. Department of Burns and Plastic Surgery, Affiliated Hospital of Jiangsu University
  7. Department of Burns and Plastic Surgery, Affiliated Hospital of Jiangnan University
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkac050
  CLC：
- Published：2023，
  
  Published Online：15 January 2023，
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Jiamin Huang, Yi Chen, Zaiwen Guo, Yanzhen Yu, Yi Zhang, Pingsong Li, Lei Shi, Guozhong Lv, Bingwei Sun, Prospective study and validation of early warning marker discovery based on integrating multi-omics analysis in severe burn patients with sepsis, Burns & Trauma, Volume 11, 2023, tkac050, https://doi.org/10.1093/burnst/tkac050
DOI：

Jiamin Huang, Yi Chen, Zaiwen Guo, Yanzhen Yu, Yi Zhang, Pingsong Li, Lei Shi, Guozhong Lv, Bingwei Sun, Prospective study and validation of early warning marker discovery based on integrating multi-omics analysis in severe burn patients with sepsis, Burns & Trauma, Volume 11, 2023, tkac050, https://doi.org/10.1093/burnst/tkac050 DOI：

摘要

Abstract

BackgroundEarly detection

timely diagnosis and rapid response are essential for case management and precautions of burn-associated sepsis. However

studies on indicators for early warning and intervention have rarely been conducted. This study was performed to better understand the pathophysiological changes and targets for prevention of severe burn injuries.MethodsWe conducted a multi-center

prospective multi-omics study

including genomics

microRNAomics

proteomics and single-cell transcriptomics

in 60 patients with severe burn injuries. A mouse model of severe burn injuries was also constructed to verify the early warning ability and therapeutic effects of potential markers.ResultsThrough genomic analysis

we identified seven important susceptibility genes (DNAH11

LAMA2

ABCA2

ZFAND4

CEP290

MUC20 and ENTPD1) in patients with severe burn injuries complicated with sepsis. Through plasma miRNAomics studies

we identified four miRNAs (hsa-miR-16-5p

hsa-miR-185-5p

hsa-miR-451a and hsa-miR-423-5p) that may serve as early warning markers of burn-associated sepsis. A proteomic study indicated the changes in abundance of major proteins at different time points after severe burn injury and revealed the candidate early warning markers S100A8 and SERPINA10. In addition

the proteomic analysis indicated that neutrophils play an important role in the pathogenesis of severe burn injuries

as also supported by findings from single-cell transcriptome sequencing of neutrophils. Through further studies on severely burned mice

we determined that S100A8 is also a potential early therapeutic target for severe burn injuries

beyond being an early warning indicator.ConclusionsOur multi-omics study identified seven susceptibility genes

four miRNAs and two proteins as early warning markers for severe burn-associated sepsis. In severe burn-associated sepsis

the protein S100A8 has both warning and therapeutic effects.

关键词

Keywords

Early warningMarkerIntegrating multi-omics analysisSevere burnSepsis

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