Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

Tiantian Zheng; Linliang Wu; Shaolan Sun; Jiawei Xu; Qi Han; Yifan Liu; Ronghua Wu; Guicai Li

doi:10.1093/burnst/tkac030

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Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

更新时间：2023-09-05

- Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration
- Burns & Trauma Vol. 10, Issue 1, (2022)
- 作者机构：
  
  1. Key laboratory of Neuroregeneration of Jiangsu and Ministry of Education
  2. Nantong University.,226001
  3. School of Medicine, Nantong University.,226001
  4. Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University
  5. National Engineering Laboratory for Modern Silk, Soochow University
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkac030
  CLC：
- Published：2022，
  
  Published Online：4 September 2022，
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Tiantian Zheng, Linliang Wu, Shaolan Sun, Jiawei Xu, Qi Han, Yifan Liu, Ronghua Wu, Guicai Li, Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration, Burns & Trauma, Volume 10, 2022, tkac030, https://doi.org/10.1093/burnst/tkac030
DOI：

Tiantian Zheng, Linliang Wu, Shaolan Sun, Jiawei Xu, Qi Han, Yifan Liu, Ronghua Wu, Guicai Li, Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration, Burns & Trauma, Volume 10, 2022, tkac030, https://doi.org/10.1093/burnst/tkac030 DOI：

摘要

Abstract

BackgroundAnisotropic topologies are known to regulate cell-oriented growth and induce cell differentiation

which is conducive to accelerating nerve regeneration

while co-culture of endothelial cells (ECs) and Schwann cells (SCs) can significantly promote the axon growth of dorsal root ganglion (DRG). However

the synergistic regulation of EC and SC co-culture of DRG behavior on anisotropic topologies is still rarely reported. The study aims to investigate the effect of anisotropic topology co-cultured with Schwann cells and endothelial cells on dorsal root ganglion behavior for promoting peripheral nerve regeneration.MethodsChitosan/artemisia sphaerocephala (CS/AS) scaffolds with anisotropic topology were first prepared using micro-molding technology

and then the surface was modified with dopamine to facilitate cell adhesion and growth. The physical and chemical properties of the scaffolds were characterized through morphology

wettability

surface roughness and component variation. SCs and ECs were co-cultured with DRG cells on anisotropic topology scaffolds to evaluate the axon growth behavior.ResultsDopamine-modified topological CS/AS scaffolds had good hydrophilicity and provided an appropriate environment for cell growth. Cellular immunofluorescence showed that in contrast to DRG growth alone

co-culture of SCs and ECs could not only promote the growth of DRG axons

but also offered a stronger guidance for orientation growth of neurons

which could effectively prevent axons from tangling and knotting

and thus may significantly inhibit neurofibroma formation. Moreover

the co-culture of SCs and ECs could promote the release of nerve growth factor and vascular endothelial growth factor

and up-regulate genes relevant to cell proliferation

myelination and skeletal development via the PI3K-Akt

MAPK and cytokine and receptor chemokine pathways.ConclusionsThe co-culture of SCs and ECs significantly improved the growth behavior of DRG on anisotropic topological scaffolds

which may provide an important basis for the development of nerve grafts in peripheral nerve regeneration.

关键词

Keywords

Schwann cellsEndothelial cellsDorsal root ganglion behaviorCo-cultureAnisotropic topologyRegulation mechanismNerve Regeneration

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