Nerve transfer with 3D-printed branch nerve conduits

Jing Zhang; Jie Tao; Hao Cheng; Haofan Liu; Wenbi Wu; Yinchu Dong; Xuesong Liu; Maling Gou; Siming Yang; Jianguo Xu

doi:10.1093/burnst/tkac010

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Nerve transfer with 3D-printed branch nerve conduits

更新时间：2022-06-09

- Nerve transfer with 3D-printed branch nerve conduits
- Burns & Trauma Vol. 10, Issue 1, (2022)
- 作者机构：
  
  1. Department of Neurosurgery
  2. Sichuan University
  3. State Key Laboratory of Biotherapy and Cancer Center
  4. Department of Stomatology
  5. Third Military Medical University
  6. Key Laboratory of Wound Repair and Regeneration of PLA
  7. Medical College of PLA
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkac010
  CLC：
- Published：2022，
  
  Published Online：15 April 2022，
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Jing Zhang, Jie Tao, Hao Cheng, Haofan Liu, Wenbi Wu, Yinchu Dong, Xuesong Liu, Maling Gou, Siming Yang, Jianguo Xu, Nerve transfer with 3D-printed branch nerve conduits, Burns & Trauma, Volume 10, 2022, tkac010, https://doi.org/10.1093/burnst/tkac010
DOI：

Jing Zhang, Jie Tao, Hao Cheng, Haofan Liu, Wenbi Wu, Yinchu Dong, Xuesong Liu, Maling Gou, Siming Yang, Jianguo Xu, Nerve transfer with 3D-printed branch nerve conduits, Burns & Trauma, Volume 10, 2022, tkac010, https://doi.org/10.1093/burnst/tkac010 DOI：

摘要

Abstract

BackgroundNerve transfer is an important clinical surgical procedure for nerve repair by the coaptation of a healthy donor nerve to an injured nerve. Usually

nerve transfer is performed in an end-to-end manner

which will lead to functional loss of the donor nerve. In this study

we aimed to evaluate the efficacy of 3D-printed branch nerve conduits in nerve transfer.MethodsCustomized branch conduits were constructed using gelatine-methacryloyl by 3D printing. The nerve conduits were characterized both in vitro and in vivo. The efficacy of 3D-printed branch nerve conduits in nerve transfer was evaluated in rats through electrophysiology testing and histological evaluation.ResultsThe results obtained showed that a single nerve stump could form a complex nerve network in the 3D-printed multibranch conduit. A two-branch conduit was 3D printed for transferring the tibial nerve to the peroneal nerve in rats. In this process

the two branches were connected to the distal tibial nerve and peroneal nerve. It was found that the two nerves were successfully repaired with functional recovery.ConclusionsIt is implied that the two-branch conduit could not only repair the peroneal nerve but also preserve partial function of the donor tibial nerve. This work demonstrated that 3D-printed branch nerve conduits provide a potential method for nerve transfer.

关键词

Keywords

Branch nerve conduitNerve transfer3D printingNerve regeneration

references

1.Mokarram N, Dymanus K, Srinivasan A, Lyon JG, Tipton J, Chu J, et al. Immunoengineering nerve repair. Proc Natl Acad Sci U S A. 2017;114:E5077–e84.

2.Dixon AR, Jariwala SH, Bilis Z, Loverde JR, Pasquina PF, Alvarez LM. Bridging the gap in peripheral nerve repair with 3D printed and bioprinted conduits. Biomaterials. 2018;186:44–63.

3.Aszmann OC, Roche AD, Salminger S, Paternostro-Sluga T, Herceg M, Sturma A, et al. Bionic reconstruction to restore hand function after brachial plexus injury: a case series of three patients. Lancet. 2015;385:2183–9.

4.Quant EC, Jeste SS, Muni RH, Cape AV, Bhussar MK, Peleg AY. The benefits of steroids versus steroids plus antivirals for treatment of Bell's palsy: a meta-analysis. BMJ. 2009;339:b3354.

5.Manni JJ, Beurskens CH, van de Velde C, Stokroos RJ. Reanimation of the paralyzed face by indirect hypoglossal-facial nerve anastomosis. Am J Surg. 2001;182:268–73.

6.Le Clerc N, Herman P, Kania R, Tran H, Altabaa K, Tran Ba Huy P, et al. Comparison of 3 procedures for hypoglossal-facial anastomosis. Otol Neurotol. 2013;34:1483–8.

7.Vincent AG, Bevans SE, Robitschek JM, Wind GG, Hohman MH. Masseteric-to-facial nerve transfer and selective Neurectomy for rehabilitation of the Synkinetic smile. JAMA Facial Plast Surg. 2019;21:504–10.

8.Biglioli F, Frigerio A, Colombo V, Colletti G, Rabbiosi D, Mortini P, et al. Masseteric-facial nerve anastomosis for early facial reanimation. J Craniomaxillofac Surg. 2012;40:149–55.

9.Bertelli JA, Ghizoni MF. A surgical approach for concomitant spinal cord and brachial plexus surgery: an anatomical study. Chir Main. 1998;17:159–64.

10.Bertelli JA, Mira JC, Pecot-Dechavassine M, Sebille A. Selective motor hyperreinnervation using motor rootlet transfer: an experimental study in rat brachial plexus. J Neurosurg. 1997;87:79–84.

11.Zhu W, Ma X, Gou M, Mei D, Zhang K, Chen S. 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol. 2016;40:103–12.

12.Wang P, Sun Y, Shi X, Shen H, Ning H, Liu H. 3D printing of tissue engineering scaffolds: a focus on vascular regeneration. Biodes Manuf. 2021;4:344–78.

13.Mei Q, Rao J, Bei HP, Liu Y, Zhao X. 3D bioprinting photo-Crosslinkable hydrogels for bone and cartilage repair. Int J Bioprint. 2021;7:367.

14.Zhu W, Tringale KR, Woller SA, You S, Johnson S, Shen H, et al. Rapid continuous 3D printing of customizable peripheral nerve guidance conduits. Mater Today (Kidlington). 2018;21:951–9.

15.Tao J, Zhang J, Du T, Xu X, Deng X, Chen S, et al. Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair. Acta Biomater. 2019;90:49–59.

16.Xu X, Tao J, Wang S, Yang L, Zhang J, Zhang J, et al. 3D printing of nerve conduits with nanoparticle-encapsulated RGFP966. Appl Mater Today. 2019;16:247–56.

17.Fairbanks BD, Schwartz MP, Bowman CN, Anseth KS. Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility. Biomaterials. 2009;30:6702–7.

18.Shirahama H, Lee BH, Tan LP, Cho NJ. Precise tuning of facile one-pot Gelatin Methacryloyl (GelMA) synthesis. Sci Rep. 2016;6:31036.

19.Gou M, Qu X, Zhu W, Xiang M, Yang J, Zhang K, et al. Bio-inspired detoxification using 3D-printed hydrogel nanocomposites. Nat Commun. 2014;5:3774.

20.Li G, Zhao X, Zhao W, Zhang L, Wang C, Jiang M, et al. Porous chitosan scaffolds with surface micropatterning and inner porosity and their effects on Schwann cells. Biomaterials. 2014;35:8503–13.

21.Wang Y, Wang W, Wo Y, Gui T, Zhu H, Mo X, et al. Orientated guidance of peripheral nerve regeneration using conduits with a microtube Array sheet (MTAS). ACS Appl Mater Interfaces. 2015;7:8437–50.

22.Huang Y, Wu W, Liu H, Chen Y, Li B, Gou Z, et al. 3D printing of functional nerve guide conduits. Burns Trauma. 2021;9:tkab011. https://doi.org/10.1093/burnst/tkab011.

23.Zhuang H, Bu S, Hua L, Darabi MA, Cao X, Xing M. Gelatin-methacrylamide gel loaded with microspheres to deliver GDNF in bilayer collagen conduit promoting sciatic nerve growth. Int J Nanomedicine. 2016;11:1383–94.

24.Hu Y, Wu Y, Gou Z, Tao J, Zhang J, Liu Q, et al. 3D-engineering of Cellularized conduits for peripheral nerve regeneration. Sci Rep. 2016;6:32184.

25.Tack P, Victor J, Gemmel P, Annemans L. 3D-printing techniques in a medical setting: a systematic literature review. Biomed Eng Online. 2016;15:115.

26.Uz M, Buyukoz M, Sharma AD, Sakaguchi DS, Altinkaya SA, Mallapragada SK. Gelatin-based 3D conduits for transdifferentiation of mesenchymal stem cells into Schwann cell-like phenotypes. Acta Biomater. 2017;53:293–306.

27.Ouyang Y, Huang C, Zhu Y, Fan C, Ke Q. Fabrication of seamless electrospun collagen/PLGA conduits whose walls comprise highly longitudinal aligned nanofibers for nerve regeneration. J Biomed Nanotechnol. 2013;9:931–43.

28.Songcharoen P. Management of brachial plexus injury in adults. Scand J Surg. 2008;97:317–23.

29.Socolovsky M, Di Masi G, Battaglia D. Use of long autologous nerve grafts in brachial plexus reconstruction: factors that affect the outcome. Acta Neurochir. 2011;153:2231–40.

30.Sarker MD, Naghieh S, McInnes AD, Schreyer DJ, Chen X. Regeneration of peripheral nerves by nerve guidance conduits: influence of design, biopolymers, cells, growth factors, and physical stimuli. Prog Neurobiol. 2018;171:125–50.

31.Zhang H, Xu J, Saijilafu. The effects of GelMA hydrogel on nerve repair and regeneration in mice with spinal cord injury. Ann Transl Med 2021;9:1147.

32.Chen J, Huang D, Wang L, Hou J, Zhang H, Li Y, et al. 3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation. J Colloid Interface Sci. 2020;574:162–73.

33.Lin YX, Li SH, Huang WC. Fabrication of soft tissue scaffold-mimicked microelectrode arrays using enzyme-mediated transfer printing. Micromachines (Basel). 2021;12:1057.

34.Ngan CGY, Quigley A, Williams RJ, O'Connell CD, Blanchard R, Boyd-Moss M, et al. Matured Myofibers in bioprinted constructs with in vivo vascularization and innervation. Gels. 2021;7:171.

35.Heo DN, Lee SJ, Timsina R, Qiu X, Castro NJ, Zhang LG. Development of 3D printable conductive hydrogel with crystallized PEDOT:PSS for neural tissue engineering. Mater Sci Eng C Mater Biol Appl. 2019;99:582–90.

36.Zhao C, Wu Z, Chu H, Wang T, Qiu S, Zhou J, et al. Thiol-rich multifunctional macromolecular Crosslinker for Gelatin-Norbornene-based bioprinting. Biomacromolecules. 2021;22:2729–39.

37.Chiu DT, Smahel J, Chen L, Meyer V. Neurotropism revisited. Neurol Res. 2004;26:381–7.

38.Abernethy DA, Thomas PK, Rud A, King RH. Mutual attraction between emigrant cells from transected denervated nerve. J Anat. 1994;184:239–49.

39.Muheremu A, Sun JG, Wang XY, Zhang F, Ao Q, Peng J. Combined use of Y-tube conduits with human umbilical cord stem cells for repairing nerve bifurcation defects. Neural Regen Res. 2016;11:664–9.

40.Shapira Y, Tolmasov M, Nissan M, Reider E, Koren A, Biron T, et al. Comparison of results between chitosan hollow tube and autologous nerve graft in reconstruction of peripheral nerve defect: an experimental study. Microsurgery. 2016;36:664–71.

41.de Ruiter GC, Spinner RJ, Verhaagen J, Malessy MJ. Misdirection and guidance of regenerating axons after experimental nerve injury and repair. J Neurosurg. 2014;120:493–501.

42.Hizay A, Ozsoy U, Demirel BM, Ozsoy O, Angelova SK, Ankerne J, et al. Use of a Y-tube conduit after facial nerve injury reduces collateral axonal branching at the lesion site but neither reduces polyinnervation of motor endplates nor improves functional recovery. Neurosurgery. 2012;70:1544–56.

43.Ozsoy U, Demirel BM, Hizay A, Ozsoy O, Ankerne J, Angelova S, et al. Hypoglossal-facial anastomosis (HFA) over a 10 mm gap bridged by a Y-tube-conduit enhances neurite regrowth and reduces collateral axonal branching at the lesion site. Restor Neurol Neurosci. 2011;29:227–42.

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