CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway

Wenwen Zhang; Yu Liao; Jiaqi Lou; Mengmeng Zhuang; Hao Yan; Qi Li; Yuequ Deng; Xiaohu Xu; Dandan Wen; Yong Sun

doi:10.1093/burnst/tkac009

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CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway

更新时间：2022-06-09

- CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway
- Burns & Trauma Vol. 10, Issue 1, (2022)
- 作者机构：
  
  1. Department of Burn Surgery, the Affiliated Huaihai Hospital of Xuzhou Medical University
  2. Department of Burn Surgery, the 71st Group Army Hospital of PLA
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkac009
  CLC：
- Published：2022，
  
  Published Online：7 March 2022，
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Wenwen Zhang, Yu Liao, Jiaqi Lou, Mengmeng Zhuang, Hao Yan, Qi Li, Yuequ Deng, Xiaohu Xu, Dandan Wen, Yong Sun, CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway, Burns & Trauma, Volume 10, 2022, tkac009, https://doi.org/10.1093/burnst/tkac009
DOI：

Wenwen Zhang, Yu Liao, Jiaqi Lou, Mengmeng Zhuang, Hao Yan, Qi Li, Yuequ Deng, Xiaohu Xu, Dandan Wen, Yong Sun, CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway, Burns & Trauma, Volume 10, 2022, tkac009, https://doi.org/10.1093/burnst/tkac009 DOI：

摘要

Abstract

BackgroundCircular RNA (circRNA) plays key regulatory roles in the development of many diseases. However the biological functions and potential molecular mechanisms of circRNA in the injury and repair of intestinal mucosa in mice after severe burns are yet to be elucidated.MethodsCell counting kit-8 (CCK-8)

5-ethynyl-2′-deoxyuridine (EdU)

wound healing and transwell assays were used to detect cell proliferation and migration ability. Real-time quantitative PCR was used to identify the expression of circRNA

microRNA and messenger RNA. Nuclear and cytoplasmic separation experiments were employed to perceive the location of circRNA_Maml2. Finally

in vitro and in vivo experiments were conducted to study the repairing effect of circRNA_Maml2 on the intestinal mucosa of mice after severe burns.ResultsWhen compared with the control group

the expression of circRNA_Maml2 was significantly reduced in the severe burn group. Furthermore

overexpression of circRNA_Maml2 promoted the proliferation and migration of CT26.wt cells in vivo and the repair of damaged intestinal mucosa in vitro. CircRNA_Maml2 acted as a sponge adsorption molecule for miR-93-3p to enhance the expression of frizzled class receptor 7 and activate the downstream Wnt/β-catenin pathway

thereby promoting the repair of the intestinal mucosa.ConclusionsOur findings demonstrate that circRNA_Maml2 regulates the miR-93-3p/FZD7/Wnt/β-catenin pathway and promotes the repair of damaged intestinal mucosa. Hence

circRNA_Maml2 is a potential therapeutic target to promote intestinal mucosal repair.

关键词

Keywords

CircRNA_Maml2Severe burnmiR-93-3pFZD7Wnt/β-catenin pathwayProliferationMigration

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