Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance
- Burns & Trauma Vol. 10, Issue 1, (2022)
- 作者机构：
1. Institute of Burns, Tongren Hospital of Wuhan University (Wuhan Third Hospital)
2. Department of Hematology
3. Peking University Shenzhen Hospital
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkac007
CLC：Published：2022，
Published Online：9 April 2022，
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Weiguo Xie, Weigang Hu, Zhuo Huang, Min Li, Hongyu Zhang, Xiaodong Huang, Paul Yao, Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance, Burns & Trauma, Volume 10, 2022, tkac007, https://doi.org/10.1093/burnst/tkac007
Weiguo Xie, Weigang Hu, Zhuo Huang, Min Li, Hongyu Zhang, Xiaodong Huang, Paul Yao, Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance, Burns & Trauma, Volume 10, 2022, tkac007, https://doi.org/10.1093/burnst/tkac007 DOI：
摘要
Abstract
BackgroundDiabetes significantly delays wound healing through oxidative stress
inflammation and impaired re-epithelialization that lead to defective regulation of the healing process
although the related mechanism remains unclear. Here
we aim to investigate the potential role and mechanism for the beneficial effect of betulinic acid (BA) on diabetic wound healing.MethodsThe molecular effect of BA on hyperglycemia-mediated gene expression
oxidative stress
inflammation and glucose uptake was evaluated in endothelial
fibroblast and muscle cells. Burn injury was introduced to streptozotocin-induced diabetic rats and BA administration through either an intraperitoneal (IP) or topical (TOP) technique was used for wound treatment. Glucose tolerance was evaluated in both muscle tissue and fibroblasts
while oxidative stress and inflammation were determined in both the circulatory system and in wound tissues. The effect of BA on the wound healing process was also evaluated.ResultsBA treatment reversed hyperglycemia-induced glucose transporter type 4 (GLUT4) suppression in both muscle and fibroblast cells. This treatment also partly reversed hyperglycemia-mediated suppression of endothelial nitric oxide synthase (eNOS)
nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and nuclear factor NFκB p65 subunit (NFκB p65) activation in endothelial cells. An in vivo rat study showed that BA administration ameliorated diabetes-mediated glucose intolerance and partly attenuated diabetes-mediated oxidative stress and inflammation in both the circulatory system and wound tissues. BA administration by both IP and TOP techniques significantly accelerated diabetic wound healing
while BA administration by either IP or TOP methods alone had a significantly lower effect.ConclusionsBA treatment ameliorates hyperglycemia-mediated glucose intolerance
endothelial dysfunction
oxidative stress and inflammation. Administration of BA by both IP and TOP techniques was found to significantly accelerate diabetic wound healing
indicating that BA could be a potential therapeutic candidate for diabetic wound healing.
关键词
Keywords
Betulinic aciddiabetesInflammationOxidative stressWound healingNuclear factor κBglucoseHyperglycemia
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