Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds

Sizhan Xia; Tingting Weng; Ronghua Jin; Min Yang; Meirong Yu; Wei Zhang; Xingang Wang; Chunmao Han

doi:10.1093/burnst/tkac001

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Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds

更新时间：2022-06-09

- Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds
- Burns & Trauma Vol. 10, Issue 1, (2022)
- 作者机构：
  
  1. Second Affiliated Hospital of Zhejiang University College of Medicine
  2. The Second Affiliated Hospital of Zhejiang University College of Medicine
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkac001
  CLC：
- Published：2022，
  
  Published Online：14 March 2022，
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Sizhan Xia, Tingting Weng, Ronghua Jin, Min Yang, Meirong Yu, Wei Zhang, Xingang Wang, Chunmao Han, Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds, Burns & Trauma, Volume 10, 2022, tkac001, https://doi.org/10.1093/burnst/tkac001
DOI：

Sizhan Xia, Tingting Weng, Ronghua Jin, Min Yang, Meirong Yu, Wei Zhang, Xingang Wang, Chunmao Han, Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds, Burns & Trauma, Volume 10, 2022, tkac001, https://doi.org/10.1093/burnst/tkac001 DOI：

摘要

Abstract

BackgroundGelatin methacryloyl (GelMA) hydrogels loaded with stem cells have proved to be an effective clinical treatment for wound healing. Advanced glycation end product (AGE)

interacting with its particular receptor (AGER)

gives rise to reactive oxygen species (ROS) and apoptosis. Curcumin (Cur) has excellent antioxidant activity and regulates intracellular ROS production and apoptosis. In this study

we developed a Cur-incorporated 3D-printed GelMA to insert into adipose-derived stem cells (ADSCs) and applied it to diabetic wounds.MethodsGelMA hydrogels with Cur were fabricated and their in vitro effects on ADSCs were investigated. We used structural characterization

western blot

ROS and apoptosis assay to evaluate the antioxidant and anti-apoptotic activity

and assessed the wound healing effects to investigate the mechanism underlying regulation of apoptosis by Cur via the AGE/AGER/nuclear factor-κB (NF-κB) p65 pathway.ResultsA 10% GelMA scaffold exhibited appropriate mechanical properties and biocompatibility for ADSCs. The circular mesh structure demonstrated printability of 10% GelMA and Cur-GelMA bioinks. The incorporation of Cur into the 10% GelMA hydrogel showed an inhibitory effect on AGEs/AGER/NF-κB p65-induced ROS generation and ADSC apoptosis. Furthermore

Cur-GelMA scaffold promoted cell survival and expedited in vivo diabetic wound healing.ConclusionsThe incorporation of Cur improved the antioxidant activity of 3D-printed GelMA hydrogel and mitigated AGE/AGER/p65 axis-induced ROS and apoptosis in ADSCs. The effects of scaffolds on wound healing suggested that Cur/GelMA-ADSC hydrogel could be an effective biological material for accelerating wound healing.

关键词

Keywords

Curcumin3D printingGelMAAdvanced glycation end productsAdipose-derived stem cellsWound healing

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