Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions

Bingmin Li; Haowen Tang; Xiaowei Bian; Kui Ma; Jiang Chang; Xiaobing Fu; Cuiping Zhang

doi:10.1093/burnst/tkab029

您当前的位置：

首页 >

文章列表页 >

Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions

更新时间：2022-06-14

- Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions
- Burns & Trauma Vol. 9, Issue 1, (2021)
- 作者机构：
  
  1. Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Division and Fourth Medical Center of Chinese PLA General Hospital
  2. Department of Dermatology
  3. Fourth Medical Center of Chinese PLA General Hospital
  4. Faculty of Hepato-Biliary-Pancreatic Surgery
  5. Chinese PLA General Hospital
  6. Shanghai Institute of Ceramics
  7. Chinese Academy of Sciences
  8. Research Unit of Trauma Care
  9. Chinese Academy of Medical Sciences
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkab029
  CLC：
- Published：2021，
  
  Published Online：30 September 2021，
扫描看全文
Bingmin Li, Haowen Tang, Xiaowei Bian, Kui Ma, Jiang Chang, Xiaobing Fu, Cuiping Zhang, Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions, Burns & Trauma, Volume 9, 2021, tkab029, https://doi.org/10.1093/burnst/tkab029
DOI：

Bingmin Li, Haowen Tang, Xiaowei Bian, Kui Ma, Jiang Chang, Xiaobing Fu, Cuiping Zhang, Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions, Burns & Trauma, Volume 9, 2021, tkab029, https://doi.org/10.1093/burnst/tkab029 DOI：

摘要

Abstract

BackgroundHuman epidermal stem cells (hESCs) play an important role in re-epithelialization and thereby in facilitating wound healing

while an effective way to activate hESCs remains to be explored. Calcium silicate (CS) is a form of bioceramic that can alter cell behavior and promote tissue regeneration. Here

we have observed the effect of CS on hESCs and investigated its possible mechanism.MethodsUsing a mouse full-thickness skin excision model

we explored the therapeutic effect of CS on wound healing and re-epithelialization. In vitro

hESCs were cultured with diluted CS ion extracts (CSIEs)

and the proliferation

migration ability and stemness of hESCs were evaluated. The effects of CS on the epidermal growth factor (EGF)

epidermal growth factor receptor (EGFR) and extracellular signal-related kinase (ERK) signaling pathway were also explored.ResultsIn vivo

CS accelerated wound healing and re-epithelialization. Immunohistochemistry demonstrated that CS upregulated cytokeratin 19 and integrin β1 expression

indicating that CS improved hESCs stemness. In vitro studies confirmed that CS improved the biological function of hESCs. And the possible mechanism could be due to the activation of the EGF/EGFR/ERK signaling pathway.ConclusionCS can promote re-epithelialization and improve the biological functions of hESCs via activating the EGF/EGFR/ERK signaling pathway.

关键词

Keywords

bioceramiccalcium silicateepidermal stem cellsre-epithelializationwounds

references

1.Gonzales KAU, Fuchs E. Skin and its regenerative powers: an alliance between stem cells and their niche. Dev Cell. 2017;43:387–401.

2.Ren S, Chen J, Duscher D, Liu Y, Guo G, Kang Y, et al. Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways. Stem Cell Res Ther. 2019;10:47.

3.Plikus MV, Gay DL, Treffeisen E, Wang A, Supapannachart RJ, Cotsarelis G. Epithelial stem cells and implications for wound repair. Semin Cell Dev Biol. 2012;23:946–53.

4.Kargozar S, Hamzehlou S, Baino F. Can bioactive glasses be useful to accelerate the healing of epithelial tissues? Korean J Couns Psychother. 2019;97:1009–20.

5.Li B, Hu W, Ma K, Zhang C, Fu X. Are hair follicle stem cells promising candidates for wound healing? Expert Opin Biol Ther. 2019;19:119–28.

6.Han Y, Li X, Zhang Y, Han Y, Chang F, Ding J. Mesenchymal stem cells for regenerative medicine. Cell. 2019;8:886.

7.Bratt-Leal AM, Carpenedo RL, Ungrin MD, Zandstra PW, McDevitt TC. Incorporation of biomaterials in multicellular aggregates modulates pluripotent stem cell differentiation. Biomaterials. 2011;32:48–56.

8.Yu Q, Chang J, Wu C. Silicate bioceramics: from soft tissue regeneration to tumor therapy. J Mater Chem B. 2019;7:5449–60.

9.Wang C, Lin K, Chang J, Sun J. Osteogenesis and angiogenesis induced by porous beta-CaSiO(3)/PDLGA composite scaffold via activation of AMPK/ERK1/2 and PI3K/Akt pathways. Biomaterials. 2013;34:64–77.

10.Katayama N, Kato H, Taguchi Y, Tanaka A, Umeda M. The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells. Int J Mol Sci. 2014;15:14026–43.

11.Zhang M, Zhang Y, Ding J, Li X, Zang C, Yin S, et al. The role of TrkA in the promoting wounding-healing effect of CD271 on epidermal stem cells. Arch Dermatol Res. 2018;310:737–50.

12.Lin K, Liu Y, Huang H, Chen L, Wang Z, Chang J. Degradation and silicon excretion of the calcium silicate bioactive ceramics during bone regeneration using rabbit femur defect model. J Mater Sci Mater Med. 2015;26:197.

13.Liu S, Jin F, Lin K, Lu J, Sun J, Chang J, et al. The effect of calcium silicate on in vitro physiochemical properties and in vivo osteogenesis, degradability and bioactivity of porous beta-tricalcium phosphate bioceramics. Biomed Mater. 2013;8:025008.

14.Yu H, Peng J, Xu Y, Chang J, Li H. Bioglass activated skin tissue engineering constructs for wound healing. ACS Appl Mater Interfaces. 2016;8:703–15.

15.Li H, Chang J. Bioactive silicate materials stimulate angiogenesis in fibroblast and endothelial cell co-culture system through paracrine effect. Acta Biomater. 2013;9:6981–91.

16.Fu X, Sun X, Li X, Sheng Z. Dedifferentiation of epidermal cells to stem cells in vivo. Lancet. 2001;358:1067–8.

17.Dekoninck S, Blanpain C. Stem cell dynamics, migration and plasticity during wound healing. Nat Cell Biol. 2019;21:18–24.

18.Takeo M, Lee W, Ito M. Wound healing and skin regeneration. Cold Spring Harb Perspect Med. 2015;5:a023267.

19.Yi M, Li H, Wang X, Yan J, Gao L, He Y, et al. Ion therapy: a novel strategy for acute myocardial infarction. Adv Sci (Weinh). 2019;6:1801260.

20.Kargozar S, Hamzehlou S, Baino F. Potential of bioactive glasses for cardiac and pulmonary tissue engineering. Materials (Basel). 2017;10:1429.

21.Wang F, Wang X, Ma K, Zhang C, Chang J, Fu X. Akermanite bioceramic enhances wound healing with accelerated reepithelialization by promoting proliferation, migration, and stemness of epidermal cells. Wound Repair Regen. 2020;28:16–25.

22.Li B, Bian X, Hu W, Wang X, Li Q, Wang F, et al. Regenerative and protective effects of calcium silicate on senescent fibroblasts induced by high glucose. Wound Repair Regen. 2020;28:315–25.

23.Veltri A, Lang C, Lien WH. Concise review: Wnt signaling pathways in skin development and epidermal stem cells. Stem Cells. 2018;36:22–35.

24.Zhang C, Chen P, Fei Y, Liu B, Ma K, Fu X, et al. Wnt/beta-catenin signaling is critical for dedifferentiation of aged epidermal cells in vivo and in vitro. Aging Cell. 2012;11:14–23.

25.Grose R, Hutter C, Bloch W, Thorey I, Watt FM, Fassler R, et al. A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair. Development. 2002;129:2303–15.

26.Moreno-Layseca P, Streuli CH. Signalling pathways linking integrins with cell cycle progression. Matrix Biol. 2014;34:144–53.

27.Guan J, Zhang J, Guo S, Zhu H, Zhu Z, Li H, et al. Human urine-derived stem cells can be induced into osteogenic lineage by silicate bioceramics via activation of the Wnt/beta-catenin signaling pathway. Biomaterials. 2015;55:1–11.

28.Kong L, Wu Z, Zhao H, Cui H, Shen J, Chang J, et al. Bioactive injectable hydrogels containing desferrioxamine and bioglass for diabetic wound healing. ACS Appl Mater Interfaces. 2018;10:30103–14.

29.Grassian AR, Schafer ZT, Brugge JS. ErbB2 stabilizes epidermal growth factor receptor (EGFR) expression via Erk and Sprouty2 in extracellular matrix-detached cells. J Biol Chem. 2011;286:79–90.

30.Harrisingh MC, Perez-Nadales E, Parkinson DB, Malcolm DS, Mudge AW, Lloyd AC. The Ras/Raf/ERK signalling pathway drives Schwann cell dedifferentiation. EMBO J. 2004;23:3061–71.

31.Hu Y, Rao SS, Wang ZX, Cao J, Tan YJ, Luo J, et al. Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function. Theranostics. 2018;8:169–84.

32.Pullar CE, Rizzo A, Isseroff RR. Beta-adrenergic receptor antagonists accelerate skin wound healing: evidence for a catecholamine synthesis network in the epidermis. J Biol Chem. 2006;281:21225–35.

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization

Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization

P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing

Topical odour management in burn patients

Obstruction of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice

Related Author

Biao Cheng

Pengcheng Xu

Yaguang Wu

Lina Zhou

Zengjun Yang

Xiaorong Zhang

Xiaohong Hu

Jiacai Yang

Related Institution

State Key Laboratory of Trauma

Department of Endocrinology

Third Military Medical University (Army Medical University)

Chongqing Key Laboratory for Disease Proteomics

Southwest Hospital

⁰