Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury
- Burns & Trauma Vol. 9, Issue 1, (2021)
- 作者机构：
1. Department of Neurology
2. Ulm University
3. Department of Neurosurgery, Kaifeng Central Hospital
4. German Center for Neurodegenerative Diseases (DZNE)
5. Institute of Anatomy and Cell Biology
6. Institute of Clinical and Experimental Trauma-Immunology
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkab027
CLC：Published：2021，
Published Online：30 September 2021，
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Zhenghui Li, Jin Zhang, Steffen Halbgebauer, Akila Chandrasekar, Rida Rehman, Albert Ludolph, Tobias Boeckers, Markus Huber-Lang, Markus Otto, Francesco Roselli, Florian olde Heuvel, Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury, Burns & Trauma, Volume 9, 2021, tkab027, https://doi.org/10.1093/burnst/tkab027
Zhenghui Li, Jin Zhang, Steffen Halbgebauer, Akila Chandrasekar, Rida Rehman, Albert Ludolph, Tobias Boeckers, Markus Huber-Lang, Markus Otto, Francesco Roselli, Florian olde Heuvel, Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury, Burns & Trauma, Volume 9, 2021, tkab027, https://doi.org/10.1093/burnst/tkab027 DOI：
摘要
Abstract
BackgroundBlood-based biomarkers have proven to be a reliable measure of the severity and outcome of traumatic brain injury (TBI) in both murine models and patients. In particular
neuron-specific enolase (NSE)
neurofilament light (NFL) and S100 beta (S100B) have been investigated in the clinical setting post-injury. Ethanol intoxication (EI) remains a significant comorbidity in TBI
with 30–40% of patients having a positive blood alcohol concentration post-TBI. The effect of ethanol on blood-based biomarkers for the prognosis and diagnosis of TBI remains unclear. In this study
we investigated the effect of EI on NSE
NFL and S100B and their correlation with blood–brain barrier integrity in a murine model of TBI.MethodsWe used ultra-sensitive single-molecule array technology and enzyme-linked immunosorbent assay methods to measure NFL
NSE
S100B and claudin-5 concentrations in plasma 3 hours post-TBI.ResultsWe showed that NFL
NSE and S100B were increased at 3 hours post-TBI. Interestingly
ethanol blood concentrations showed an inverse correlation with NSE but not with NFL or S100B. Claudin-5 levels were increased post-injury but no difference was detected compared to ethanol pretreatment. The increase in claudin-5 post-TBI was correlated with NFL but not with NSE or S100B.ConclusionsEthanol induces an effect on biomarker release in the bloodstream that is different from TBI not influenced by alcohol. This could be the basis of investigations into humans.
关键词
Keywords
Traumatic brain injuryEthanolBiomarkersneurofilament lightneuron-specific enolaseS100BClaudin-5
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