Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles

Yijie Zhang; Enhejirigala; Bin Yao; Zhao Li; Wei Song; Jianjun Li; Dongzhen Zhu; Yuzhen Wang; Xianlan Duan; Xingyu Yuan; Sha Huang; Xiaobing Fu

doi:10.1093/burnst/tkab013

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Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles

更新时间：2022-06-14

- Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles
- Burns & Trauma Vol. 9, Issue 1, (2021)
- 作者机构：
  
  1. Research Center for Tissue Repair and Regeneration
  2. Chinese PLA General Hospital and PLA Medical College
  3. PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury
  4. College of Graduate, Tianjin Medical University
  5. Institute of Basic Medical Research, Inner Mongolia Medical University
  6. The Shenzhen Key Laboratory of Health Sciences and Technology
  7. Tsinghua University
  8. Department of General Surgery
  9. Chinese PLA General Hospital
  10. Department of Burn and Plastic Surgery
  11. School of Medicine, Nankai University
  12. Research Unit of Trauma Care
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkab013
  CLC：
- Published：2021，
  
  Published Online：4 May 2021，
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Yijie Zhang, Enhejirigala, Bin Yao, Zhao Li, Wei Song, Jianjun Li, Dongzhen Zhu, Yuzhen Wang, Xianlan Duan, Xingyu Yuan, Sha Huang, Xiaobing Fu, Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles, Burns & Trauma, Volume 9, 2021, tkab013, https://doi.org/10.1093/burnst/tkab013
DOI：

Yijie Zhang, Enhejirigala, Bin Yao, Zhao Li, Wei Song, Jianjun Li, Dongzhen Zhu, Yuzhen Wang, Xianlan Duan, Xingyu Yuan, Sha Huang, Xiaobing Fu, Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles, Burns & Trauma, Volume 9, 2021, tkab013, https://doi.org/10.1093/burnst/tkab013 DOI：

摘要

Abstract

BackgroundSweat glands (SGs) and hair follicles (HFs) are two important cutaneous appendages that play crucial roles in homeostatic maintenance and thermoregulation

and their interaction is involved in wound healing. SGs can be regenerated from mesenchymal stem cell-laden 3D bioprinted scaffolds

based on our previous studies

whereas regeneration of HFs could not be achieved in the same model. Due to the lack of an in vitro model

the underlying molecular mechanism of the interaction between SGs and HFs in regeneration could not be fully understood. The purpose of the present study was to establish an in vitro model of skin constructs with SGs and HFs and explore the interaction between these two appendages in regeneration.MethodsTo investigate the interaction effects between SGs and HFs during their regeneration processes

a combined model was created by seeding HF spheroids on 3D printed SG scaffolds. The interaction between SG scaffolds and HF spheroids was detected using RNA expression and immunofluorescence staining. The effects of microenvironmental cues on SG and HF regeneration were analysed by altering seed cell types and plantar dermis homogenate in the scaffold.ResultsAccording to this model

we overcame the difficulties in simultaneously inducing SG and HF regeneration and explored the interaction effects between SG scaffolds and HF spheroids. Surprisingly

HF spheroids promoted both SG and HF differentiation in SG scaffolds

while SG scaffolds promoted SG differentiation but had little effect on HF potency in HF spheroids. Specifically

microenvironmental factors (plantar dermis homogenate) in SG scaffolds effectively promoted SG and HF genesis in HF spheroids

no matter what the seed cell type in SG scaffolds was

and the promotion effects were persistent.ConclusionsOur approach elucidated a new model for SG and HF formation in vitro and provided an applicable platform to investigate the interaction between SGs and HFs in vitro. This platform might facilitate 3D skin constructs with multiple appendages and unveil the spatiotemporal molecular program of multiple appendage regeneration.

关键词

Keywords

Skin regenerationSweat glandsHair follicle3D bioprintingSpheroid cultureSkin constructs

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