Obstruction of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice
- Burns & Trauma Vol. 9, Issue 1, (2021)
- 作者机构：
1. State Key Laboratory of Trauma
2. Institute of Burn Research
3. Chongqing Key Laboratory for Disease Proteomics
4. Department of Dermatology
5. Army Military Medical University
6. Department of Osteopathic Medicine
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkab004
CLC：Published：2021，
Published Online：29 April 2021，
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Yunxia Chen, Xiaorong Zhang, Zhihui Liu, Jiacai Yang, Cheng Chen, Jue Wang, Zengjun Yang, Lei He, Pengcheng Xu, Xiaohong Hu, Gaoxing Luo, Weifeng He, Obstruction of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice, Burns & Trauma, Volume 9, 2021, tkab004, https://doi.org/10.1093/burnst/tkab004
Yunxia Chen, Xiaorong Zhang, Zhihui Liu, Jiacai Yang, Cheng Chen, Jue Wang, Zengjun Yang, Lei He, Pengcheng Xu, Xiaohong Hu, Gaoxing Luo, Weifeng He, Obstruction of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice, Burns & Trauma, Volume 9, 2021, tkab004, https://doi.org/10.1093/burnst/tkab004 DOI：
摘要
Abstract
BackgroundDelayed wound healing remains a common but challenging problem in patients with acute or chronic wound following accidental scald burn injury. However
the systematic and detailed evaluation of the scald burn injury
including second-degree deep scald (SDDS) and third-degree scald (TDS)
is still unclear. The present study aims to analyze the wound-healing speed
the formation of granulation tissue
and the healing quality after cutaneous damage.MethodsIn order to assess SDDS and TDS
the models of SDDS and TDS were established using a scald instrument in C57BL/6 mice. Furthermore
an excisional wound was administered on the dorsal surface in mice (Cut group). The wound-healing rate was first analyzed at days 0
3
5
7
15 and 27
with the Cut group as a control. Then
on the full-thickness wounds
hematoxylin and eosin (H&E) staining
Masson staining
Sirius red staining
Victoria blue staining and immunohistochemistry were performed to examine re-epithelialization
the formation of granulation tissue
vascularization
inflammatory infiltration and the healing quality at different time points in the Cut
SDDS and TDS groups.ResultsThe presented data revealed that the wound-healing rate was higher in the Cut group
when compared with the SDDS and TDS groups. H&E staining showed that re-epithelialization
formation of granulation tissue and inflammatory infiltration were greater in the Cut group
when compared with the SDDS and TDS groups. Immunohistochemistry revealed that the number of CD31
vascular endothelial growth factor A
transforming growth factor-β and α-smooth muscle actin reached preferential peak in the Cut group
when compared with other groups. In addition
Masson staining
Sirius red staining
Victoria blue staining
Gordon-Sweets staining and stress analysis indicated that the ratio of collagen I to III
reticular fibers
failure stress
Young’s modulus and failure length in the SDDS group were similar to those in the normal group
suggesting that healing quality was better in the SDDS group
when compared with the Cut and TDS groups.ConclusionOverall
the investigators first administered a comprehensive analysis in the Cut
SDDS and TDS groups through in vivo experiments
which further proved that the obstacle of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice.
关键词
Keywords
Burn wound healingSecond-degree deep scaldThird-degree scaldre-epithelializationGranulation tissueHealing quality
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