Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization

Pengcheng Xu; Yaguang Wu; Lina Zhou; Zengjun Yang; Xiaorong Zhang; Xiaohong Hu; Jiacai Yang; Mingying Wang; Binjie Wang; Gaoxing Luo; Weifeng He; Biao Cheng

doi:10.1093/burnst/tkaa028

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Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization

更新时间：2022-06-09

- Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization
- Burns & Trauma Vol. 8, Issue 1, (2020)
- 作者机构：
  
  1. Department of Burn and Plastic Surgery
  2. Department of Dermatology
  3. Third Military Medical University (Army Medical University)
  4. Department of Endocrinology
  5. State Key Laboratory of Trauma
  6. Institute of Burn Research
  7. Southwest Hospital
  8. Chongqing Key Laboratory for Disease Proteomics
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkaa028
  CLC：
- Published：2020，
  
  Published Online：14 August 2020，
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Pengcheng Xu, Yaguang Wu, Lina Zhou, Zengjun Yang, Xiaorong Zhang, Xiaohong Hu, Jiacai Yang, Mingying Wang, Binjie Wang, Gaoxing Luo, Weifeng He, Biao Cheng, Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization, Burns & Trauma, Volume 8, 2020, tkaa028, https://doi.org/10.1093/burnst/tkaa028
DOI：

Pengcheng Xu, Yaguang Wu, Lina Zhou, Zengjun Yang, Xiaorong Zhang, Xiaohong Hu, Jiacai Yang, Mingying Wang, Binjie Wang, Gaoxing Luo, Weifeng He, Biao Cheng, Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization, Burns & Trauma, Volume 8, 2020, tkaa028, https://doi.org/10.1093/burnst/tkaa028 DOI：

摘要

Abstract

BackgroundAutologous platelet-rich plasma (PRP) has been suggested to be effective for wound healing. However

evidence for its use in patients with acute and chronic wounds remains insufficient. The aims of this study were to comprehensively examine the effectiveness

synergy and possible mechanism of PRP-mediated improvement of acute skin wound repair.MethodsFull-thickness wounds were made on the back of C57/BL6 mice. PRP or saline solution as a control was administered to the wound area. Wound healing rate

local inflammation

angiogenesis

re-epithelialization and collagen deposition were measured at days 3

7 and 14 after skin injury. The biological character of epidermal stem cells (ESCs)

which reflect the potential for re-epithelialization

was further evaluated in vitro and in vivo.ResultsPRP strongly improved skin wound healing

which was associated with regulation of local inflammation

enhancement of angiogenesis and re-epithelialization. PRP treatment significantly reduced the production of inflammatory cytokines interleukin-17A and interleukin-1β. An increase in the local vessel intensity and enhancement of re-epithelialization were also observed in animals with PRP administration and were associated with enhanced secretion of growth factors such as vascular endothelial growth factor and insulin-like growth factor-1. Moreover

PRP treatment ameliorated the survival and activated the migration and proliferation of primary cultured ESCs

and these effects were accompanied by the differentiation of ESCs into adult cells following the changes of CD49f and keratin 10 and keratin 14.ConclusionPRP improved skin wound healing by modulating inflammation and increasing angiogenesis and re-epithelialization. However

the underlying regulatory mechanism needs to be investigated in the future. Our data provide a preliminary theoretical foundation for the clinical administration of PRP in wound healing and skin regeneration.

关键词

Keywords

Wound healingPlatelet-rich plasmaInflammationre-epithelializationAngiogenesisCollagen depositionepidermal stem cells

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