Metal chelation reduces skin epithelial inflammation and rescues epithelial cells from toxicity due to thermal injury in a rat model
- Burns & Trauma Vol. 8, Issue 1, (2020)
- 作者机构：
1. Department of Surgery, University of Texas Medical Branch
2. Department of Biochemistry and Molecular Biology, University of Texas Medical Branch
3. Department of Anesthesiology, University Blvd301., University of Texas Medical Branch
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkaa024
CLC：Published：2020，
Published Online：2 October 2020，
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Amina El Ayadi, Cheng Z Wang, Min Zhang, Michael Wetzel, Anesh Prasai, Celeste C Finnerty, Perenlei Enkhbaatar, David N Herndon, Naseem H Ansari, Metal chelation reduces skin epithelial inflammation and rescues epithelial cells from toxicity due to thermal injury in a rat model, Burns & Trauma, Volume 8, 2020, tkaa024, https://doi.org/10.1093/burnst/tkaa024
Amina El Ayadi, Cheng Z Wang, Min Zhang, Michael Wetzel, Anesh Prasai, Celeste C Finnerty, Perenlei Enkhbaatar, David N Herndon, Naseem H Ansari, Metal chelation reduces skin epithelial inflammation and rescues epithelial cells from toxicity due to thermal injury in a rat model, Burns & Trauma, Volume 8, 2020, tkaa024, https://doi.org/10.1093/burnst/tkaa024 DOI：
摘要
Abstract
BackgroundOne of the most pervasive complications of burn injury is wound progression
characterized by continuous tissue destruction in untreated wounds
which leads to wound infection
inflammation
oxidative stress and excessive scar formation. We determined whether additional tissue destruction could be attenuated with Livionex formulation (LF) lotion
which contains a metal-chelating agent and reduces inflammation in burn wounds.MethodsWe subjected male Sprague Dawley rats to a 2% total body surface area (TBSA) burn using a brass comb model and topically applied LF lotion (containing ethylenediaminetetraacetic acid and methyl sulfonyl methane) to the affected area every 8 hours over 3 days. Inflammatory cytokine levels
cell apoptosis and wound healing were compared in LF lotion-treated and untreated rats. Statistical analysis was performed using a one-way analysis of variance in conjunction with Tukey’s post-hoc test.ResultsSerum inflammatory cytokines were not detectable after 3 days
suggesting that small burn wounds induce only an immediate
localized inflammatory response. Microscopy revealed that LF lotion improved burn site pathology. Deoxynucleotidyl transferase biotin-d-UTP nick-end labeling staining showed reduced cell death in the LF-treated samples. LF lotion prevented the spread of tissue damage
as seen by increased amounts of Ki-67-positive nuclei in the adjacent epidermis and hair follicles. Tumor necrosis factor-alpha
interleukin-6 and inducible nitric oxide synthase levels in LF-treated skin sections from burned rats were comparable to the levels observed in unburned control sections
indicating that LF lotion reduces inflammation in and around the burn site.ConclusionsThese results establish LF lotion as a therapeutic agent for reducing inflammatory stress
cell death and tissue destruction when applied immediately after a burn injury. Further studies of LF lotion on large TBSA burns will determine its efficacy as an emergency treatment for reducing long-term morbidity and scarring.
关键词
Keywords
Burn progressionCytokinesOxidative stressCell deathWound healingInflammationMetal chelationskin
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