Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury

Jiro Oba; Motonori Okabe; Toshiko Yoshida; Chika Soko; Moustafa Fathy; Koji Amano; Daisuke Kobashi; Masahiro Wakasugi; Hiroshi Okudera

doi:10.1093/burnst/tkaa014

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Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury

更新时间：2022-06-09

- Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury
- Burns & Trauma Vol. 8, Issue 1, (2020)
- 作者机构：
  
  1. Department of Emergency and Disaster Medicine
  2. University of Toyama
  3. Department of Regenerative Medicine
  4. Department of Biochemistry
  5. Minia University
  6. Department of Emergency Surgery
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkaa014
  CLC：
- Published：2020，
  
  Published Online：27 July 2020，
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Jiro Oba, Motonori Okabe, Toshiko Yoshida, Chika Soko, Moustafa Fathy, Koji Amano, Daisuke Kobashi, Masahiro Wakasugi, Hiroshi Okudera, Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury, Burns & Trauma, Volume 8, 2020, tkaa014, https://doi.org/10.1093/burnst/tkaa014
DOI：

Jiro Oba, Motonori Okabe, Toshiko Yoshida, Chika Soko, Moustafa Fathy, Koji Amano, Daisuke Kobashi, Masahiro Wakasugi, Hiroshi Okudera, Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury, Burns & Trauma, Volume 8, 2020, tkaa014, https://doi.org/10.1093/burnst/tkaa014 DOI：

摘要

Abstract

Background Severe burn injuries create large skin defects that render the host susceptible to bacterial infections. Burn wound infection often causes systemic sepsis and severe septicemia

resulting in an increase in the mortality of patients with severe burn injuries. Therefore

appropriate wound care is important to prevent infection and improve patient outcomes. However

it is difficult to heal a third-degree burn injury. The aim of this study was to investigate whether hyperdry human amniotic membrane (HD-AM) could promote early granulation tissue formation after full-thickness skin excision in third-degree burn injury sites in mice.Methods After the development of HD-AM and creation of a third-degree burn injury model

the HD-AM was either placed or not placed on the wound area in the HD-AM group or HD-AM group

respectively. The groups were prepared for evaluation on postoperative days 1

4 and 7. Azan staining was used for granulation tissue evaluation

and estimation of CD163

transforming growth factor beta-1 (TGF-β1)

vascular endothelial growth factor (VEGF)

CD31

alpha-smooth muscle actin (α-SMA) and Iba1 expression was performed by immunohistochemical staining. Quantitative reverse-transcription polymerase chain reaction (PCR) was used to investigate gene expression of growth factors

cell migration chemokines and angiogenic and inflammatory markers.ResultsThe HD-AM group showed significant early and qualitatively good growth of granulation tissue on the full-thickness skin excision site. HD-AM promoted early-phase inflammatory cell infiltration

fibroblast migration and angiogenesis in the granulation tissue. Additionally

the early infiltration of cells of the immune system was observed.Conclusions HD-AM may be useful as a new wound dressing material for full-thickness skin excision sites after third-degree burn injuries

and may be a new therapeutic technique for improving the survival rate of patients with severe burn injuries.

关键词

Keywords

Hyperdry human amniotic membraneThird-degree burnGranulationCell migrationInflammationAngiogenesis

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