Adipose-derived stem cells improve grafted burn wound healing by promoting wound bed blood flow
- Burns & Trauma Vol. 8, Issue 1, (2020)
- 作者机构：
1. Department of Anesthesiology
2. Department of Surgery
3. Shriners Hospitals for Children,Galveston
4. Sealy Center for Molecular Medicine
5. Center for Biomedical Engineering
6. Department of Neuroscience
- 作者简介：
- 基金信息：
DOI：10.1093/burnst/tkaa009
CLC：Published：2020，
Published Online：21 April 2020，
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Osamu Fujiwara, Anesh Prasai, Dannelys Perez-Bello, Amina El Ayadi, Irene Y Petrov, Rinat O Esenaliev, Yuriy Petrov, David N Herndon, Celeste C Finnerty, Donald S Prough, Perenlei Enkhbaatar, Adipose-derived stem cells improve grafted burn wound healing by promoting wound bed blood flow, Burns & Trauma, Volume 8, 2020, tkaa009, https://doi.org/10.1093/burnst/tkaa009
Osamu Fujiwara, Anesh Prasai, Dannelys Perez-Bello, Amina El Ayadi, Irene Y Petrov, Rinat O Esenaliev, Yuriy Petrov, David N Herndon, Celeste C Finnerty, Donald S Prough, Perenlei Enkhbaatar, Adipose-derived stem cells improve grafted burn wound healing by promoting wound bed blood flow, Burns & Trauma, Volume 8, 2020, tkaa009, https://doi.org/10.1093/burnst/tkaa009 DOI：
摘要
Abstract
BackgroundResearchers have explored the use of adipose-derived stem cells (ASCs) as a cell-based therapy to cover wounds in burn patients; however
underlying mechanistic aspects are not completely understood. We hypothesized that ASCs would improve post-burn wound healing after eschar excision and grafting by increasing wound blood flow via induction of angiogenesis-related pathways.MethodsTo test the hypothesis
we used an ovine burn model. A 5 cm2 full thickness burn wound was induced on each side of the dorsum. After 24 hours
the burned skin was excised and a 2 cm2 patch of autologous donor skin was grafted. The wound sites were randomly allocated to either topical application of 7 million allogeneic ASCs or placebo treatment (phosphate-buffered saline [PBS]). Effects of ASCs culture media was also compared to those of PBS. Wound healing was assessed at one and two weeks following the application of ASCs. Allogeneic ASCs were isolated
cultured and characterized from non-injured healthy sheep. The identity of the ASCs was confirmed by flow cytometry analysis
differentiation into multiple lineages and gene expression via real-time polymerase chain reaction. Wound blood flow
epithelialization
graft size and take and the expression of vascular endothelial growth factor (VEGF) were determined via enzyme-linked immunosorbent assay and Western blot.ResultsTreatment with ASCs accelerated the patch graft growth compared to the control (p < 0.05). Topical application of ASCs significantly increased wound blood flow (p < 0.05). Expression of VEGF was significantly higher in the wounds treated with ASCs compared to control (p < 0.05).ConclusionsASCs accelerated grafted skin growth possibly by increasing the blood flow via angiogenesis induced by a VEGF-dependent pathway.
关键词
Keywords
Wound healingBurnAdipose-derived stem cellsAngiogenesisVascular endothelial growth factorVEGFblood flow
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