JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA <em>MALAT1</em>

Junping Liu; Yutong Shi; Minmin Wu; Fengmei Zhang; Mengqi Xu; Zhiqiang He; Min Tang

doi:10.1016/j.gendis.2022.07.006

您当前的位置：

首页 >

文章列表页 >

JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1

Full-length article | 更新时间：2023-08-29

- JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1
- Genes & Diseases Vol. 10, Issue 5, (2023)
- 作者机构：
  
  Key Laboratory of Laboratory Medical Diagnostics Designated by the Chinese Ministry of Education, Chongqing Medical University,Chongqing,China,400016
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.07.006
  CLC：
- Published：2023，
  
  Published Online：1 September 2023，
扫描看全文
Junping Liu, Yutong Shi, Minmin Wu, et al. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1. [J]. Genes & Diseases 10(5).(2023)
DOI：

Junping Liu, Yutong Shi, Minmin Wu, et al. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1. [J]. Genes & Diseases 10(5).(2023) DOI： 10.1016/j.gendis.2022.07.006.

摘要

Abstract

Despite significant improvements in five-year survival rates due to early diagnosis and combination therapy

triple-negative breast cancer (TNBC) treatment remains a major challenge. Finding new and effective targets for diagnosis and drug therapy is urgent for TNBC patients. Jagged-1 (JAG1)

one of the canonical ligands of the Notch signaling pathway

is involved in vascular budding and is a poor prognostic factor of TNBC. In this study

combined with quantitative real-time PCR

database analysis

animal experiments

and other means

JAG1 was confirmed to be related to the poor prognosis of TNBC patients.

JAG1

was highly expressed in MDA-MB-231 Bone (231B) cells

with stronger invasion and metastasis ability than MDA-MB-231 (231) cells. Treatment of human vascular endothelial cells (HUVEC) with TNBC conditioned medium showed that TNBC JAG1 promoted the angiogenesis of HUVEC. Next

we detected the exosomes extracted from TNBC conditioned medium and found that JAG1 promoted the exosome secretion from 231 cells via ALIX-RAB11A/RAB35. In addition

we also found that the exosomes from JAG1 overexpressed TNBC cells contained more long non-coding RNA (lncRNA)

MALAT1

and

MALAT1

promoted angiogenesis of HUVEC by targeting

miR-140-5p

. Finally

the angiogenesis-promoting effect of JAG1 in TNBC was further investigated by matrix gel assay. In conclusion

we reveal that JAG1 has a pro-invasion effect on TNBC and is involved in microenvironment angiogenesis by promoting exosome secretion and the MALAT1-miR-140-5p-JAG1/VEGFA pathway.

关键词

Keywords

AngiogenesisExosomeJAG1lncRNAMALAT1Triple-negative breast cancer

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The journey towards physiology and pathology: Tracing the path of neuregulin 4

HIF-1α signaling: Essential roles in tumorigenesis and implications in targeted therapies

Mechanistic insight into lysyl oxidase in vascular remodeling and angiogenesis

LncRNA H19 mediates BMP9-induced angiogenesis in mesenchymal stem cells by promoting the p53-Notch1 angiogenic signaling axis

Defective EMC1 drives abnormal retinal angiogenesis via Wnt/β-catenin signaling and may be associated with the pathogenesis of familial exudative vitreoretinopathy

Related Author

Liang Guo

Wangjing Mu

Hongyang Luo

Jieying Zhu

Min Chen

Yan Zhao

Cheng Xing

Yating Deng

Related Institution

School of Exercise and Health and Shanghai Frontiers Science Research Base of Exercise and Metabolic Health, Shanghai University of Sport

Hunan Engineering Research Center of Cell Immunotherapy for Hematopoietic Malignancies, The Second Xiangya Hospital, Central South University

Hunan Key Laboratory of Tumor Models and Individualized Medicine, The Second Xiangya Hospital, Central South University

Department of Hematology, The Second Xiangya Hospital, Central South University

Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education

⁰

JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1

DOI：10.1016/j.gendis.2022.07.006

摘要

Abstract

关键词

Keywords

references