Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

Yunyi Liu; Juan Li; Hailong Ou; Dan Qi; Bei Hu; Yuxi Xu; Jian Hu; Yi Xiong; Luling Xia; Jason H. Huang; Xiaoxiao Hu; Erxi Wu

doi:10.1016/j.gendis.2022.07.002

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Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

Full-length article | 更新时间：2023-08-29

- Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma
- Genes & Diseases Vol. 10, Issue 5, (2023)
- 作者机构：
  
  1. Texas A & M University School of Medicine, College Station,TX,USA,77843
  2. Department of Neurosurgery and Neuroscience Institute, Baylor Scott & White Health, Temple,TX,USA,76508
  3. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan University,Hunan,Changsha,China,410082
  4. Department of Respiratory Medicine, The Third Xiangya Hospital, Central South University,Hunan,Changsha,China,410013
  5. LIVESTRONG Cancer Institutes and Department of Oncology, Dell Medical School, The University of Texas at Austin,TX,Austin,USA,78712
  6. Greater Bay Area Institute for Innovation, Hunan University,Guangdong,Guangzhou,China,511300
  7. Texas A & M University School of Pharmacy, College Station,TX,USA,77843
  8. Shenzhen Research Institute, Hunan University,Guangdong,Shenzhen,China,518000
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.07.002
  CLC：
- Published：2023，
  
  Published Online：1 September 2023，
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Yunyi Liu, Juan Li, Hailong Ou, et al. Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma. [J]. Genes & Diseases 10(5).(2023)
DOI：

Yunyi Liu, Juan Li, Hailong Ou, et al. Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma. [J]. Genes & Diseases 10(5).(2023) DOI： 10.1016/j.gendis.2022.07.002.

摘要

Abstract

ptamers

short single DNA or RNA oligonucleotides

have shown immense application potential as molecular probes for the early diagnosis and therapy of cancer. However

conventional cell-SELEX technologies for aptamer discovery are time-consuming and laborious. Here we discovered a new aptamer BC-3 by using an improved rapid X-Aptamer selection process for human bladder carcinoma

for which there is no specific molecular probe yet. We show that BC-3 exhibited excellent affinity in bladder cancer cells but not normal cells. We demonstrate that BC-3 displayed high selectivity for tumor cells over their normal counterparts

in vitro

in mice

and in patient tumor tissue specimens. Further endocytosis pathway analysis revealed that BC-3 internalized into bladder cancer cells via clathrin-mediated endocytosis. Importantly

we identified ribosomal protein S7 (RPS7) as the binding target of BC-3 via an integrated methodology (mass spectrometry

colocalization assay

and immunoblotting). Together

we report that a novel aptamer BC-3 is discovered for bladder cancer and its properties in the disease are unearthed. Our findings will facilitate the discovery of novel diagnostic and therapeutic strategies for bladder cancer.

关键词

Keywords

Bladder cancerCell-SELEXClathrin-mediated endocytosisIntracellular colocalizationRibosomal protein S7X-aptamer selections

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