Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis

Tingwei Zhang; Ziqi Chen; Mengyuan Zhu; Xuan Jing; Xiaohui Xu; Xulei Yuan; Mengjiao Zhou; Yanan Zhang; Miao Lu; Duanjing Chen; Shihan Xu; Jinlin Song

doi:10.1016/j.gendis.2022.06.009

您当前的位置：

首页 >

文章列表页 >

Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis

Full-length article | 更新时间：2023-08-29

- Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis
- Genes & Diseases Vol. 10, Issue 4, (2023)
- 作者机构：
  
  Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory for Oral Biomedical Engineering of Higher Education, and Stomatological Hospital of Chongqing Medical University,Chongqing,China,401147
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.06.009
  CLC：
- Published：2023，
  
  Published Online：1 July 2023，
扫描看全文
Tingwei Zhang, Ziqi Chen, Mengyuan Zhu, et al. Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis. [J]. Genes & Diseases 10(4).(2023)
DOI：

Tingwei Zhang, Ziqi Chen, Mengyuan Zhu, et al. Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis. [J]. Genes & Diseases 10(4).(2023) DOI： 10.1016/j.gendis.2022.06.009.

摘要

Abstract

Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have emerged as a new mode of intercellular crosstalk and are responsible for many of the therapeutic effects of MSCs. To promote the application of MSC-EVs

recent studies have focused on the manipulation of MSCs to improve the production of EVs and EV-mediated activities. The current paper details an optimization method using non-invasive low-intensity pulsed ultrasound (LIPUS) as the stimulation for improving oral MSC-EV production and effectiveness. Stem cells from apical papilla (SCAP)

a type of oral mesenchymal stem cell

displayed intensity-dependent pro-osteogenic and anti-inflammatory responses to LIPUS without significant cytotoxicity or apoptosis. The stimuli increased the secretion of EVs by promoting the expression of neutral sphingomyelinases in SCAP. In addition

EVs from LIPUS-induced SCAP exhibited stronger efficacy in promoting the osteogenic differentiation and anti-inflammation of periodontal ligament cells

in vitro

and alleviating oral inflammatory bone loss

in vivo

. In addition

LIPUS stimulation affected the physical characteristics and miRNA cargo of SCAP-EVs. Further investigations indicated that miR-935 is an important mediator of the pro-osteogenic and anti-inflammatory capabilities of LIPUS-induced SCAP-EVs. Taken together

these findings demonstrate that LIPUS is a simple and effective physical method to optimize SCAP-EV production and efficacy.

关键词

Keywords

Extracellular vesiclesLow-intensity pulsed ultrasoundOral bone lossPeriodontitisStem cells from apical papilla

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of extracellular vesicle-derived noncoding RNAs on pre-metastatic niche and tumor progression

Advances in oral mesenchymal stem cell-derived extracellular vesicles in health and disease

Emerging chemical engineering of exosomes as “bioscaffolds” in diagnostics and therapeutics

Circulating and non-circulating proteins and nucleic acids as biomarkers and therapeutic molecules in ovarian cancer

The miR-21-5p enriched in the apoptotic bodies of M2 macrophage-derived extracellular vesicles alleviates osteoarthritis by changing macrophage phenotype

Related Author

Zhuang Chen

Qiming Wang

Jinbo Liu

Wenkang Wang

Weitang Yuan

Yang Liu

Zhenqiang Sun

Chengzeng Wang

Related Institution

The First Affiliated Hospital of Zhengzhou University

Department of Radiotherapy, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital

Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University

Department of Internal Medicine, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital

Academy of Medical Sciences of Zhengzhou University

⁰