PGC-1α promotes mitochondrial respiration and biogenesis during the differentiation of hiPSCs into cardiomyocytes

Qin Zhou; Hao Xu; Liang Yan; Liang Ye; Xinyuan Zhang; Bin Tan; Qin Yi; Jie Tian; Jing Zhu

doi:10.1016/j.gendis.2020.12.006

您当前的位置：

首页 >

文章列表页 >

PGC-1α promotes mitochondrial respiration and biogenesis during the differentiation of hiPSCs into cardiomyocytes

更新时间：2022-06-24

- PGC-1α promotes mitochondrial respiration and biogenesis during the differentiation of hiPSCs into cardiomyocytes
- Genes & Diseases Vol. 8, Issue 6, (2021)
- 作者机构：
  
  1. Department of Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders (Chongqing) China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’s Hospital of Chongqing Medical University,Chongqing,PR China,400000
  2. Chongqing Key Laboratory of Pediatrics,Chongqing,PR China,400000
  3. Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China,Sichuan Province,Chengdu,PR China,610073
  4. Department of Clinical Laboratory Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders (Chongqing) China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’s Hospital of Chongqing Medical University,Chongqing,PR China,400000
  5. Department of Cardiovascular (Internal Medicine), Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders (Chongqing) China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’s Hospital of Chongqing Medical University,Chongqing,PR China,400000
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2020.12.006
  CLC：
- Published：2021，
  
  Published Online：1 November 2021，
扫描看全文
Qin Zhou, Hao Xu, Liang Yan, et al. PGC-1α promotes mitochondrial respiration and biogenesis during the differentiation of hiPSCs into cardiomyocytes. [J]. Genes & Diseases 8(6).(2021)
DOI：

Qin Zhou, Hao Xu, Liang Yan, et al. PGC-1α promotes mitochondrial respiration and biogenesis during the differentiation of hiPSCs into cardiomyocytes. [J]. Genes & Diseases 8(6).(2021) DOI： 10.1016/j.gendis.2020.12.006.

摘要

Abstract

lthough it is widely accepted that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are readily available

robustly reproducible

and physiologically appropriate human cells for clinical applications and research in the cardiovascular field

hiPSC-CMs cultured

in vitro

retain an immature metabolic phenotype that limits their application

and little is known about the underlying molecular mechanism controlling mitochondrial metabolic maturation during human induced pluripotent stem cells (hiPSCs ) differentiation into cardiomyocytes. In this study

we found that peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) played an important role in inducing mitochondrial biogenesis and establishing oxidative phosphorylation (OXPHOS) during the cardiac differentiation of hiPSCs. Knocking down PGC-1α by siRNA impaired mitochondrial respiration

while upregulating PGC-1α by ZLN005 promoted mitochondrial biosynthesis and function by regulating the expression of downstream genes involved in mitochondrial dynamics and oxidative metabolism in hiPSC-CMs. Furthermore

we found that estrogen-related receptor α (ERRα) was required for the induction of PGC-1α stimulatory effects in hiPSC-CMs. These findings provide key insights into the molecular control of mitochondrial metabolism during cardiac differentiation and may be used to generate more metabolically mature cardiomyocytes for application.

关键词

Keywords

Cardiac differentiationhiPSCsMitochondrial metabolismPGC-1αMaturation

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

PTEN-induced putative kinase 1 regulates mitochondrial quality control and is essential for the maturation of human induced pluripotent stem cell-derived cardiomyocytes

Related Author

Huiwen Liu

Yanting Sun

Hao Xu

Bin Tan

Qin Yi

Jie Tian

Jing Zhu

Related Institution

Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University

Department of Pediatric Research Institute, Children's Hospital of Chongqing Medical University

Department of Cardiovascular (Internal Medicine), Children's Hospital of Chongqing Medical University

Chongqing Key Laboratory of Pediatrics

⁰