Human obstructive (postvasectomy) and nonobstructive azoospermia – Insights from scRNA-Seq and transcriptome analysis

Shitao Chen; Geng An; Hanshu Wang; Xiaolong Wu; Ping Ping; Longfei Hu; Yunmei Chen; Jue Fan; C. Yan Cheng; Fei Sun

doi:10.1016/j.gendis.2020.09.004

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Human obstructive (postvasectomy) and nonobstructive azoospermia – Insights from scRNA-Seq and transcriptome analysis

更新时间：2022-06-24

- Human obstructive (postvasectomy) and nonobstructive azoospermia – Insights from scRNA-Seq and transcriptome analysis
- Genes & Diseases Vol. 9, Issue 3, (2022)
- 作者机构：
  
  1. International Peace Maternity and Child Health Hospital, Shanghai Key Laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University,Shanghai,PR China,200030
  2. Department of Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University,Guangdong,Guangzhou,PR China,510150
  3. Institute of Reproductive Medicine, School of Medicine, Nantong University,Jiangsu,Nantong,PR China,226001
  4. Department of Urology, Shanghai Human Sperm Bank, Renji Hospital, School of Medicine, Shanghai Jiao Tong University,Shanghai,PR China,200120
  5. Singleron Biotechnologies Ltd,Jiangsu,Nanjing,PR China,210000
  6. The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council,NY,New York,USA,10065
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2020.09.004
  CLC：
- Published：2022，
  
  Published Online：1 May 2022，
扫描看全文
Shitao Chen, Geng An, Hanshu Wang, et al. Human obstructive (postvasectomy) and nonobstructive azoospermia – Insights from scRNA-Seq and transcriptome analysis. [J]. Genes & Diseases 9(3).(2022)
DOI：

Shitao Chen, Geng An, Hanshu Wang, et al. Human obstructive (postvasectomy) and nonobstructive azoospermia – Insights from scRNA-Seq and transcriptome analysis. [J]. Genes & Diseases 9(3).(2022) DOI： 10.1016/j.gendis.2020.09.004.

摘要

Abstract

substantial number of male infertility is caused by azoospermia. However

the underlying etiology and the molecular basis remain largely unknown. Through single-cell (sc)RNA sequencing

we had analyzed testis biopsy samples from two patients with obstructive azoospermia (OA) and nonobstructive azoospermia (NOA). We found only somatic cells in the NOA samples and explored the transcriptional changes in Sertoli cells in response to a loss of interactions with germ cells. Moreover

we observed a germ cell population discrepancy between an OA (postvasectomy) patient and a healthy individual. We confirmed this observation in a secondary study with two datasets at GSM3526588 and GSE124263 for detailed analysis wherein the regulatory mechanisms at the transcriptional level were identified. These findings thus provide valuable information on human spermatogenesis

and we also identified insightful information for further research on reproduction-related diseases.

关键词

Keywords

Androgen signalingRetinoic acidSertoli cell-only syndromeSingle-cellSpermatogenesis

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