Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p

Yun Bai; Xiaoshan Gong; Rui Dong; Zhen Cao; Ce Dou; Chuan Liu; Jianmei Li; Fei Kang; Jingjin Dai; Chunrong Zhao; Zhansong Tian; Jiulin Tan; Qijie Dai; Shiwu Dong

doi:10.1016/j.gendis.2020.07.013

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Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p

更新时间：2022-06-24

- Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p
- Genes & Diseases Vol. 9, Issue 2, (2022)
- 作者机构：
  
  1. Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University,Chongqing,PR China,400038
  2. Institute of Trauma Orthopedics, Army General Hospital of PLA,Beijing,PR China,100700
  3. Department of Orthopedics, Southwest Hospital, Third Military Medical University,Chongqing,PR China,400038
  4. State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University,Chongqing,PR China,400038
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2020.07.013
  CLC：
- Published：2022，
  
  Published Online：1 March 2022，
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Yun Bai, Xiaoshan Gong, Rui Dong, et al. Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p. [J]. Genes & Diseases 9(2).(2022)
DOI：

Yun Bai, Xiaoshan Gong, Rui Dong, et al. Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p. [J]. Genes & Diseases 9(2).(2022) DOI： 10.1016/j.gendis.2020.07.013.

摘要

Abstract

Endochondral bone formation is an important route for bone repair. Although emerging evidence has revealed the functions of long non-coding RNAs (lncRNAs) in bone and cartilage development

the effect of lncRNAs in endochondral bone repair is still largely unknown. Here

we identified a lncRNA

named Hypertrophic Chondrocyte Angiogenesis-related lncRNA (HCAR)

and proved it to promote the endochondral bone repair by upregulating the expression of matrix metallopeptidase 13 (Mmp13) and vascular endothelial growth factor α (Vegfa) in hypertrophic chondrocytes. Lnc-HCAR knockdown in hypertrophic chondrocytes restrained the cartilage matrix remodeling and decrease the CD31

Emcn

vessels number in a bone repair model. Mechanistically

we proved that lnc-HCAR was mainly enriched in the cytoplasm using fluorescence in situ hybridization (FISH) assay

and it acted as a molecular sponge for miR-15b-5p. Further

in hypertrophic chondrocytes

lnc-HCAR competitively bound to miR-15b-5p to increase Vegfa and Mmp13 expression. Our results proved that lncRNA is deeply involved in endochondral bone repair

which will provide a new theoretical basis for future strategies for promoting fracture healing.

关键词

Keywords

Bone marrow mesenchymal stem cellsBone repairChondrocyteEnchondral bone repairLong non coding RNA

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