Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1α expression participates in neural restoration in hypoxic-ischemic brain damage rats

Zhou Xiaoqin; Liu Jia; Dai Mengjie; Gu Jialu; Bi Yang; Wang Yuting; Hu Huajian; Liu Bo; Zhang Xiaojun; Li Zhongyue; Chen Jie; Li Tingyu; Zhan Xue

doi:10.1016/j.gendis.2020.01.012

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Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1α expression participates in neural restoration in hypoxic-ischemic brain damage rats

更新时间：2022-06-24

- Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1α expression participates in neural restoration in hypoxic-ischemic brain damage rats
- Genes & Diseases Vol. 8, Issue 3, (2021)
- 作者机构：
  
  1. Department of Gastroenterology, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing),PR China,401122
  2. International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University,Chongqing,PR China,401122
  3. Department of Neonatology, Chongqing Health Center for Women and Children,PR China,400021
  4. Child Health Centre of Northwest Women and Children's Hospital,USA
  5. Department of Pediatric Research Institute, Chongqing Key Laboratory of Child Health and Nutrition, Children's Hospital of Chongqing Medical University,Chongqing,PR China,401122
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2020.01.012
  CLC：
- Published：2021，
  
  Published Online：1 May 2021，
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Zhou Xiaoqin, Liu Jia, Dai Mengjie, et al. Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1α expression participates in neural restoration in hypoxic-ischemic brain damage rats. [J]. Genes & Diseases 8(3).(2021)
DOI：

Zhou Xiaoqin, Liu Jia, Dai Mengjie, et al. Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1α expression participates in neural restoration in hypoxic-ischemic brain damage rats. [J]. Genes & Diseases 8(3).(2021) DOI： 10.1016/j.gendis.2020.01.012.

摘要

Abstract

The transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) can promote hypoxic-ischemic brain damage (HIBD) nerve repair

but finding suitable seed cells to optimize transplantation and improve treatment efficiency is an urgent problem to be solved. In this study

we induced hUC-MSCs into dedifferentiated hUC-MSCs (De-hUC-MSCs)

and the morphology

stem cell surface markers

proliferation and tri-directional differentiation ability of the De-hUC-MSCs and hUC-MSCs were detected. A whole-gene chip was utilized for genome cluster

gene ontology and KEGG pathway analyses of differentially expressed genes. De-hUC-MSCs were transplanted into HIBD rats

and behavioral experiments and immunofluorescence assays were used to assess the therapeutic effect. A lentivirus vector for human stromal cell-derived factor-1 (hSDF-1α) was constructed

and the role of hSDF-1α in the neuroprotective effect and mechanism of De-hUC-MSCs was verified. De-hUC-MSCs displayed similar cell morphology

stem cell surface marker expression

cell proliferation and even three-dimensional differentiation ability as hUC-MSCs but exhibited greater treatment potential

in vivo

. The reprogramming mechanism of hSDF-1α participated in the dedifferentiation process. By successfully constructing a stable hSDF-1α cell line

we found that De-hUC-MSCs might participate in nerve repair through the hSDF-1α/CXCR4/PI3K/Akt pathway. De-hUC-MSCs reprogramming of endogenous hSDF-1α expression may mediate the hSDF-1α/CXCR4/PI3K/Akt pathway involved in nerve repair in HIBD rats.

关键词

Keywords

DedifferentiationHuman umbilical cord mesenchymal stem cellsHypoxic-ischemic brain damageNeurorestorationReprogrammingStromal cell-derived factor-1

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