All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members

Jiejie Cui; Mengjia Gong; Shuyu Fang; Chaoqun Hu; Yi Wang; Jingfang Zhang; Ni Tang; Yun He

doi:10.1016/j.gendis.2019.12.012

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All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members

更新时间：2022-06-24

- All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members
- Genes & Diseases Vol. 8, Issue 4, (2021)
- 作者机构：
  
  1. Department of Pediatric Surgery, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University,Chongqing,PR China,401122
  2. Puyang People's Hospital,Henan Province,Puyang,PR China,457000
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2019.12.012
  CLC：
- Published：2021，
  
  Published Online：1 July 2021，
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Jiejie Cui, Mengjia Gong, Shuyu Fang, et al. All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members. [J]. Genes & Diseases 8(4).(2021)
DOI：

Jiejie Cui, Mengjia Gong, Shuyu Fang, et al. All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members. [J]. Genes & Diseases 8(4).(2021) DOI： 10.1016/j.gendis.2019.12.012.

摘要

Abstract

a potential chemo-therapeutic agent

all-trans retinoic acid (ATRA) can significantly reverse epithelial-mesenchymal transition (EMT) of hepal-6 hepatocarcinoma cell line

in vitro

but the mechanism is unclear. The expression profile of microRNA-200 (miR-200) families is different in hepatocellular carcinoma. In this study

we found that ATRA treatment could up-regulate the expression of miR-200a-3p

200c-3p

and 141-3p

which were involved in ATRA regulated proliferation and apoptosis of hepal-6 cell

but not colony formation. Meanwhile

miR-200a-3p

200c-3p

and 141-3p could recovery ATRA inhibited migration and invasion abilities of hepal-6 cells at various levels. miR-200a-3p and 200c-3p prevented ATRA from inducing the differentiation and hepatic functions of hepal-6 cells. Antagomir specific for miR-200a-3p and 200c-3p down-regulated the expression of CK18

but only miR-200a-3p antagomir played prominent role in regulating the expression of these mesenchymal markers

N-Cadherin

Snail and Twist. The transcriptional activities of 8 transcription factors were up-regulated and 35 transcription factors were down-regulated by ATRA. Compared with ATRA group

inhibition of miR-200a-3p

200c-3p

and 141-3p significantly strengthened the expression of Fra1/Jun (AP1)

Ets1/PEA3

Brn3

and Zeb1/AREB6 at varying degrees. Therefore

this result suggested that ATRA may suppress EMT through down-regulating miR-200a-3p

200c-3p and 141-3p related transcription factors. miR-200 and their downstream genes might be the potentially specific targets for the treatment of hepatocarcinoma.

关键词

Keywords

All-trans retinoic acidDifferentiationEpithelial–mesenchymal transitionHepatocellular carcinoma cellsmicroRNA 200

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