Tetrandrine inhibits proliferation of colon cancer cells by BMP9/ PTEN/ PI3K/AKT signaling

Ya Zhou; Li Mu; Xiao-Lu Liu; Qin Li; Li-Xuan Ding; Hong-Chuan Chen; Ying Hu; Fu-Shu Li; Wen-Juan Sun; Bai-Cheng He; Ke Wu

doi:10.1016/j.gendis.2019.10.017

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Tetrandrine inhibits proliferation of colon cancer cells by BMP9/ PTEN/ PI3K/AKT signaling

更新时间：2022-06-24

- Tetrandrine inhibits proliferation of colon cancer cells by BMP9/ PTEN/ PI3K/AKT signaling
- Genes & Diseases Vol. 8, Issue 3, (2021)
- 作者机构：
  
  1. Department of Pharmacology, School of Pharmacy, Chongqing Medical University,Chongqing,PR China,400016
  2. Key Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University,Chongqing,PR China,400016
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2019.10.017
  CLC：
- Published：2021，
  
  Published Online：1 May 2021，
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Ya Zhou, Li Mu, Xiao-Lu Liu, et al. Tetrandrine inhibits proliferation of colon cancer cells by BMP9/ PTEN/ PI3K/AKT signaling. [J]. Genes & Diseases 8(3).(2021)
DOI：

Ya Zhou, Li Mu, Xiao-Lu Liu, et al. Tetrandrine inhibits proliferation of colon cancer cells by BMP9/ PTEN/ PI3K/AKT signaling. [J]. Genes & Diseases 8(3).(2021) DOI： 10.1016/j.gendis.2019.10.017.

摘要

Abstract

Despite advances in screening and treatment

colon cancer remains one of the leading causes of cancer-related death. Finding novel and useful drug treatment targets is also an urgent need for clinical applications. Tetrandrine (Tet) is extracted from the Chinese medicinal herbal medicine

which is a well-known calcium blocker with a variety of pharmacological activities

including anti-cancer. In this study

we recruited cell viability assay

flow cytometry analysis

cloning formation to confirm that Tet can inhibit the proliferation of SW620 cells

and induce apoptosis. Mechanically

we confirmed that Tet up-regulates the mRNA and protein level of BMP9 in SW620 cells. Over-expression BMP9 enhances the anti-cancer effects of Tet in SW620 cells

but these effects can be partly reversed by silencing BMP9. Also

Tet reduces phosphorylation of Aktl/2/3 in SW620 cells

which could be elevated by overexpressed BMP9 and impaired by silencing BMP9. Furthermore

we demonstrated that Tet reduces phosphorylated PTEN

which can be promoted by overexpressed BMP9

analogously also be attenuated through silencing BMP9. Finally

we introduced a xenograft tumor model to investigate the anti-proliferative effect of Tet

further to explore the effects of BMP9 and PTEN in SW620 cells. Our findings suggested that the anti-cancer activity of Tet in SW620 cells may be mediated partly by up-regulating BMP9

followed by inactivation PI3K/Akt through up-regulating PTEN at least.

关键词

Keywords

Akt1/2/3BMP9Colon cancerPTENTetrandrine (Tet)

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