Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic osteomyelitis. We have previously identified that this material can effectively treat chronic osteomyelitis caused by

Staphylococcus aureus

in vivo

. However

the potential mechanisms of antibacterial and osteogenic induction properties remain unclear. Thus

for osteogenesis property

immunohistochemistry

PCR

and Western blot were performed to detect the expression of osteogenic markers. Furthermore

flow cytometry and TUNEL were applied to analyze MC3T3-E1 proliferation and apoptosis. For antibacterial property

the material was co-cultivated with bacteria

bacterial colony forming units was counted and the release time of the effective levofloxacin was assayed by agar disc-diffusion test. Moreover

scanning electron microscope was applied to observe adhesion of bacteria. In terms of osteogenic induction

we found BMSCs adherently grew more prominently on Lev/MSNs/n-HA/PU. Lev/MSNs/n-HA/PU also enhanced the expression of osteogenic markers including OCN and COL1α1

as well as effectively promoted the transition from G1 phase to G2 phase. Furthermore

Lev/MSNs/n-HA/PU could reduce apoptosis of MC3T3-E1. Besides

both Lev/MSNs/n-HA/PU and n-HA/PU materials could inhibit bacterial colonies

while Lev/MSNs/n-HA/PU possessed a stronger antibacterial activities

and lower bacterial adhesion than n-HA/PU. These results illustrated that Lev/MSNs/n-HA/PU composite scaffold possess favorable compatibility

in vitro

which induce osteogenic differentiation of MSCs

promote proliferation and differentiation of MC3T3-E1

and inhibit apoptosis. Moreover

clear

in vitro

antibacterial effect of Lev/MSNs/n-HA/PU was also observed. In summary

this study replenishes the potential of Lev/MSNs/n-HA/PU composite scaffold possess dual functions of anti-infection and enhanced osteogenesis for future clinical application.