High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma

Yang Xiong; Sen Hu; Hongyao Zhou; Hui Zeng; Xuan He; Dongni Huang; Xiaoyu Li

doi:10.1016/j.gendis.2019.03.006

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High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma

更新时间：2022-06-24

- High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma
- Genes & Diseases Vol. 7, Issue 2, (2020)
- 作者机构：
  
  1. Department of the First Clinical Medicine, Chongqing Medical University,Chongqing,China,400016
  2. College of Stomatology, Chongqing Medical University,Chongqing,China,401331
  3. Department of the Second Clinical Medicine, Chongqing Medical University,Chongqing,China,400016
  4. Department of Obstetrics, First Affiliated Hospital, Chongqing Medical University,Chongqing,China,400016
  5. Laboratory of Innovation, Basic Medical Experimental Teaching Center, Chongqing Medical University,Chongqing,China,401331
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2019.03.006
  CLC：
- Published：2020，
  
  Published Online：1 June 2020，
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Yang Xiong, Sen Hu, Hongyao Zhou, et al. High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma. [J]. Genes & Diseases 7(2).(2020)
DOI：

Yang Xiong, Sen Hu, Hongyao Zhou, et al. High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma. [J]. Genes & Diseases 7(2).(2020) DOI： 10.1016/j.gendis.2019.03.006.

摘要

Abstract

decrease in microbial infection in adolescents is implicated with an increase in the incidence of asthma and allergic diseases in adulthood

indicating that the microbiome plays a critical role in asthma. However

the microbial composition of the lower respiratory tract remains unclear

hindering the further exploration of the pathogenesis of asthma. This study aims to explore the microbial distribution and composition in the lungs of normal rats and rats with allergic asthma via 16S rDNA sequencing.The DNA of the pulmonary microbiome was extracted from the left lungs collected from normal control group (NC)

saline control group (SC)

and allergic asthma group (AA) under aseptic conditions. After the 16s rDNA V4V5 region was amplified

the products were sequenced using Illumina high-throughput technology and subjected to operational taxonomic unit (OTU) cluster and taxonomy analysis.The OTU values of AA increased significantly compared with those of NC and SC. Microbiome structure analysis showed that the dominant phylum of the pulmonary microbiome changed from

Proteobacteria

in NC to

Firmicutes

in AA. Linear discriminant analysis indicated that the key microbiomes involved in the three groups varied.Numerous microbiomes stably settled in the lungs of the rats in NC and AA. The structure and diversity of the pulmonary microbiome in AA differed from those in NC.

关键词

Keywords

16S rDNAAllergic asthmaIllumina sequencingPulmonary microbiomeRats

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