The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients

Hongzhan Zhang; Chunyu Huang; Xian Chen; Longfei Li; Su Liu; Yuye Li; Yongnu Zhang; Yong Zeng; Lina Hu

doi:10.1016/j.gendis.2019.03.005

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The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients

更新时间：2022-06-24

- The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients
- Genes & Diseases Vol. 7, Issue 2, (2020)
- 作者机构：
  
  1. Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University,Chongqing,PR China,400010
  2. Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital,Guangdong,Shenzhen,PR China,518045
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2019.03.005
  CLC：
- Published：2020，
  
  Published Online：1 June 2020，
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Hongzhan Zhang, Chunyu Huang, Xian Chen, et al. The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients. [J]. Genes & Diseases 7(2).(2020)
DOI：

Hongzhan Zhang, Chunyu Huang, Xian Chen, et al. The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients. [J]. Genes & Diseases 7(2).(2020) DOI： 10.1016/j.gendis.2019.03.005.

摘要

Abstract

Natural killer (NK) cells are thought to play a key role in the successful establishment of a pregnancy by facilitating immunological adaptation of the semi-allogeneic developing embryo. The aim of this study was to explore the cell number

immunophenotypic characteristics

and activities of peripheral blood NK cells in women with repeated implantation failure (RIF). Peripheral blood was obtained from 27 women with RIF and 11 healthy

fertile controls during the middle luteal phase of the menstrual cycle. CD3

CD56

NK cells were quantified and analyzed by flow cytometry for the expression of cytolytic molecules (granzyme B

granulysin

and perforin) as well as cell surface receptors responsible for NK cell activation or inhibition (NKG2D

NKp30

NKp46

CD158a

CD158b). NK cytotoxicity was measured at three effector-to-target cell ratios. Women with RIF and fertile controls did not differ significantly in the percentage of circulating CD3

−

CD56

NK cells

or in the proportions of these cells that expressed granzyme B

granulysin

or perforin. The two groups also did not differ significantly in the proportions of NK cells expressing the receptors NKG2D

NKp30

NKp46

CD158a or CD158b. General linear model analysis showed that NK cytotoxicity increased with effector-to-target cell ratio. However

NK cytotoxicity did not differ significantly between patients with RIF and fertile controls. These results suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.

关键词

Keywords

Activating receptorInhibitory receptorNK cytotoxicityPeripheral blood NK cellsRepeated implantation failure

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