Objective     To explore the effect of LPS on the expression and phosphorylation of Akt

a PTEN/PI3k signal transduction downstream molecule

in vivo

and

in vitro

.      Methods     RAW264.7 cells were attacked with LPS at different concentrations. The Akt protein expression and phosphorylation level were detected by Western blotting at 8

24

48 h after attack. Six h after receiving 20 mg/kg LPS intra-peritoneal injection

the mice were killed and their lung tissues were resected for Akt protein expression and phosphorylation.      Results     The Akt protein expression of RAW264.7 cells showed no obvious difference after LPS attack at different doses

but their phosphorylation l

evel was increased obviously with the increase of the doses. Whereas the phosphorylation level of Akt protein was decreased in the lung tissue of mice 6 h after LPS attack.      Conclusion     The phosphorylation level of Akt protein is different after LPS attack

in vitro

and

in vivo

. The Akt protein phosphorylation is increased obviously

in vitro

but decreased in mice model of endotoxemia.