Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance

TENG Xiao-chun; LIU Hai-feng; FANG Dian-chun; YANG Shi-ming; WANG Guo-an; CHEN Gang

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Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance

更新时间：2025-01-15

- Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance
- Journal of Army Medical University Vol. 29, Issue 3, Pages: 240-242(2007)
- 作者机构：
  
  1. 第三军医大学西南医院全军消化内科中心
  2. 武警总医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.2;R735.2;R394-33
- Published：2007，
  
  Published Online：15 February 2007，
- 稿件说明：
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TENG XIAO-CHUN, LIU HAI-FENG, FANG DIAN-CHUN, et al. Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance. [J]. Journal of army medical university, 2007, 29(3): 240-242.
DOI：

TENG XIAO-CHUN, LIU HAI-FENG, FANG DIAN-CHUN, et al. Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance. [J]. Journal of army medical university, 2007, 29(3): 240-242. DOI：

摘要

目的探讨Na

-H

交换泵-1（Na

-H

exchanger1，NHE-1）反义基因转染对人胃癌细胞内pH值(intracellular pH

pHi)的调节作用及其在肿瘤基因治疗中的价值。方法构建人NHE-1基因反义真核表达载体

采用脂质体法将其转染至SGC-7901胃癌细胞中

采用荧光探针检测转染前后细胞内pH值的变化以及对细胞增殖和凋亡的影响。结果转染反义基因的细胞pHi明显降低，增殖能力降低，细胞凋亡率增加。结论 NHE-1基因的表达在肿瘤细胞pHi及增殖和凋亡调节中起重要作用。

Abstract

Objective To study the effects of the transefection of antisense human Na

-H

exchanger 1 (NHE-1) on the pHi of SGC-7901 and its potential role in the gene therapy for cancers. Methods Eukaryotic expression vector pcDNA3.1 encoding antisense NHE-1 was constructed with recombinant DNA technique and transfected into SGC-7901 with liposome DOTAP. The pHi changes of the transfected cells and untransfected cells was detected with a fluorescent probe

BCECF. The proliferation and apoptosis of the cells was studied with flow cytometry. Results Antisense NHE-1 gene transfection induced the acidification

decreased proliferation

and elevated apoptosis of SGC-7901 cells. Concl

usion The results suggest that NHE-1 gene plays an important role in the regulation of pHi

reproduction and apoptosis of tumor cells. Inhibiting the over-expression and activity of NHE-1 may be of important clinical value.

关键词

NHE1基因真核表达载体反义基因治疗胃癌

Keywords

Na+-H+ exchanger 1eukaryotic expression vectorantisense gene therapygastric cancer

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