Research on optimal culture condition for influenza virus in different cells

FAN Dong-ying; CHEN Jun-ying; ZHANG Xin-wen; LI Wei-dong; LIAO Guo-yang

您当前的位置：

首页 >

文章列表页 >

Research on optimal culture condition for influenza virus in different cells

更新时间：2025-01-15

- Research on optimal culture condition for influenza virus in different cells
- Journal of Army Medical University Vol. 29, Issue 6, Pages: 510-512(2007)
- 作者机构：
  
  中国医学科学院中国协和医科大学医学生物学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q939.405;R373.13
- Published：2007，
  
  Published Online：30 March 2007，
- 稿件说明：
移动端阅览
FAN DONG-YING, CHEN JUN-YING, ZHANG XIN-WEN, et al. Research on optimal culture condition for influenza virus in different cells. [J]. Journal of army medical university, 2007, 29(6): 510-512.
DOI：

FAN DONG-YING, CHEN JUN-YING, ZHANG XIN-WEN, et al. Research on optimal culture condition for influenza virus in different cells. [J]. Journal of army medical university, 2007, 29(6): 510-512. DOI：

摘要

目的探索流感病毒在细胞中培养的适宜条件。方法采用MEK细胞、FRhK-4细胞、MDCK细胞分别接种甲型流感病毒H1N1型、H3N2型和乙型流感病毒疫苗毒株，观察3种毒株在不同培养条件下的细胞病变、血凝滴度。结果 3株流感病毒接种细胞在33 ℃培养72 h后出现拉网、圆缩、脱落等现象；FRhK-4细胞和MDCK细胞对流感病毒较敏感。胞龄为48 h的细胞，在NaHCO

的终浓度为1.4‰，TPCK-胰酶浓度为1.0 μg/ml的病毒维持液培养流感病毒为最适条件。结论寻找到了细胞流感病毒的适宜条件。

Abstract

Objective To search for optimal culture condition of influenza virus in the tender cells. Methods Influenza A (H3N2

H1N1) and B virus were inoculated in MDCK cells

MEK cells and FRhK-4 cells under different culture conditions to observe cytopathic effect (CPE)

hemagglutination titer. Results after incubated at 33 ℃ for 72 h

cytopathic effect of viral infection in all three kinds of cells was observed

and hemagglutination assay showed MDCK cells and FRhK-4 cells were more sensitive than MEK cells to influenza virus. It is the best culture condition that the cells aged 48 h in maintenance media containing 1.4‰ NaHCO

1.0 μg/ml trypsin-TPCK. Conclusion The optimal culture conditions for influenza virus in cells were showed in this study.

关键词

流感病毒细胞培养血凝滴度

Keywords

influenza viruscell culturehemagglutination titer

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Primary culture and transfection of trophoblastic cells

Distribution, isolation and culture of epidermal stem cells of rats in vitro

Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro

Comparison of different culture systems for mouse lymphatic endothelial cells in vitro

Influence of ectopic and normal endometrial stromal cells on GJIC in epithelial cells in vitro

Related Author

XU Yuan-jiu

JIANG Hong-yan

CAI Yan

WANG Ying-xiong

WENG Ya-guang

WANG Jian

ZHU Dan

SHI Qiong

Related Institution

Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Key Laboratory of Molecular Biology on Infectious Disease, Ministry of Education, Chongqing Medical University

Institute of Bioengineering, Chongqing University

Department of Cell Biology, College of Medicine, Third Military Medical University

Department of Plastic Surgery, Southwest Hospital, Third Millitary Medical University

Cancer Research Center，Xinqiao Hospital，Third Military Medical University

⁰