Improvement of primary culture methods for human endometrial cells

CHEN Cheng; CHANG Qing; LIANG Zhi-qing; AI Guo-ping; ZHANG Li-long

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Improvement of primary culture methods for human endometrial cells

更新时间：2025-01-15

- Improvement of primary culture methods for human endometrial cells
- Journal of Army Medical University Vol. 29, Issue 6, Pages: 507-509(2007)
- 作者机构：
  
  第三军医大学：西南医院妇产科，军事预防医学院防原医学教研室，全军复合伤研究所，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.65;R329.33
- Published：2007，
  
  Published Online：30 March 2007，
- 稿件说明：
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CHEN CHENG, CHANG QING, LIANG ZHI-QING, et al. Improvement of primary culture methods for human endometrial cells. [J]. Journal of army medical university, 2007, 29(6): 507-509.
DOI：

CHEN CHENG, CHANG QING, LIANG ZHI-QING, et al. Improvement of primary culture methods for human endometrial cells. [J]. Journal of army medical university, 2007, 29(6): 507-509. DOI：

摘要

目的寻求一种简便高效的人子宫内膜上皮细胞和间质细胞分离、纯化与鉴定方法，建立稳定的子宫内膜细胞体外培养体系。方法简化Ryan等的子宫内膜细胞原代培养方法的取材，分离和培养条件，分离出高纯度的子宫内膜上皮细胞和间质细胞进行体外培养和传代。结果培养成功的子宫内膜上皮细胞与间质细胞均可稳定传代，体外生长10～50 d，纯度均达90％以上。结论该方法简便高效，可建立稳定的人子宫内膜细胞体外培养体系。

Abstract

Objective To explore the methods to isolate

purify and identify human endometrial cells

establish the stable endometrial cells culture system

in vitro

. Methods Highly purified human endometrial epithelial cells and stromal cells were obtained by improved Ryan’s procedure for specimen preparation

isolation and culture ocnditions. Results The endometrial epithelial cells and stromal cells could be passaged stably and survived 10-50 days

in vitro

and their purity was above 90%. Conclusion The improved primary culture methods for human endometrial cells is convenient and of high performance. The stable human endometrial cells culture system

in vitro

is established

providing cell platform for studying the physiologic and pathologic mechanism of human endometrium and the drug action mechanism on human endom

etrium.

关键词

子宫内膜细胞原代培养改良

Keywords

endometrial cellsprimary cultureimprovement

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LI Qing-jun

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LIANG Zhi-qing

Related Institution

河北医科大学基础医学院病理生理学教研室

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Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University

Department of Surgical Applied Anatomy and Operative Surgery, College of Basic Medical Sciences, Third Military Medical University

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