Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H<sub>3</sub>N<sub>2</sub>

WANG Huan; WU Zhong-ming; MA Rui; WANG Yu

您当前的位置：

首页 >

文章列表页 >

Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H₃N₂

更新时间：2025-01-15

- Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H₃N₂
- Journal of Army Medical University Vol. 29, Issue 3, Pages: 219-221(2007)
- 作者机构：
  
  遵义医学院基础医学部微生物学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R373.13;R73-362;R737-33
- Published：2007，
  
  Published Online：15 February 2007，
- 稿件说明：
移动端阅览
WANG HUAN, WU ZHONG-MING, MA RUI, et al. Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H₃N₂. [J]. Journal of army medical university, 2007, 29(3): 219-221.
DOI：

WANG HUAN, WU ZHONG-MING, MA RUI, et al. Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H₃N₂. [J]. Journal of army medical university, 2007, 29(3): 219-221. DOI：

摘要

目的探讨甲型流感病毒H

对人子宫内膜腺癌细胞系(endometrial adenocarcinoma cell lines，JEC)细胞的凋亡诱导作用及机制。方法取不同血凝单位的H

感染JEC细胞，经HE染色、琼脂糖凝胶电泳以及流式细胞术等方法检测细胞凋亡情况，并用免疫细胞化学法检测H

对JEC细胞Fas、FasL及TGF-β表达的影响。结果感染后的JEC在形态上呈现凋亡变化；电泳出现梯状条带；流式细胞术显示细胞凋亡率随病毒感染时间的延长而降低，且有随着病毒浓度增加而增高的趋势。免疫细胞化学法示：病毒感染后JEC细胞的Fas染色增强，TGF-β染色减弱，但FasL始终没有表达。结论甲型流感病毒H

能够诱导JEC细胞凋亡，此作用与病毒浓度及感染时间有一定关系，其机制可能与Fas和TGF-β的表达有关。

Abstract

Objective To study the apoptosis-inducing effects and mechanisms of influenza virus A H

on a self-established endometrial adenocarcinoma cell line JEC. Methods JEC cells were infected with different concentrations of H

the apoptosis were detected using the HE staining

DNA agarose gel electrophoresis and flow cytometry. The expressions of Fas

FasL and TGF-β were examined by immunocytochemical staining. Results After infection

the JEC cells showed the morpholog

ical apoptosis; DNA agarose electrophoresis demonstrated a ladder-like pattern of DNA fragments; FITC/PI stained FCM showed the apoptotic rate of JEC had been decreased along with the prolonging of infected time and elevated with the increase of the virus concentrations. Immunocytochemical staining showed that enhanced expression of Fas

attenuated expression of TGF-β

and no expression of FasL after H

infection. Conclusion Influenza virus A H

can induce JEC cells to apoptosis in time- and concentration- dependent manners

which may be related with the expressions of Fas and TGF-β.

关键词

甲型流感病毒细胞凋亡人子宫内膜腺癌细胞系

Keywords

influenza virus Aapoptosisendometrial adenocarcinoma cells

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

LI Zhiqiang

JIANG Xiuxing

HU Jinjiao

DING Xin

LEI Ling

GAO Ning

LEI Ling

JIANG Xiuxing

Related Institution

Department of Pharmacognosy and Traditional Chinese Medicine, Faculty of Pharmacy and Laboratory Medicine, Army Medical University (Third Military Medical University)

Department of Pharmacognosy and Traditional Chinese Medicine, Faculty of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University)

School of Public Health, Xinxiang Medical University

Institute of Toxicology, 3Department of Environmental Health, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

School of Public Health, China Medical University

⁰

Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H3N2

DOI：

摘要

Abstract

关键词

Keywords

references

Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H₃N₂