Objectives     To explore the optimal dosage and evaluate the safety of energy controllable steep pulse (ECSP) in the treatment of implanted rabbit tumor.      Methods     The models of VX

2

breast carcinoma were established in 10 New England rabbits

and then the animals were given ECSP treatment under orthogonality designed dosages of ECSP. The volumes of necrosis tumors were measured by TTC staining. The best dosage was analyzed by intuitionistic analysis. The models of VX

2

liver carcinoma were established in 20 New England rabbits. The normal tissues surrounding the tumor were extracted

the DNA fragmentation

analysis and comet assay were carried out.      Results     Voltage took the priority among the 4 parameters

and for best dosage they were 150 V for voltage

100 Hz for frequency

0.01μm for pulse width

and 15 min for time if the interaction of these 4 factors was not considered. The electrophoresis of DNA in the peripheral normal tissue was an integrity straining after ECSP treatment. No comet tailing was observed

indicating no DNA lesions after ECSP treatment.      Conclusion     The best dosage is 150 V for voltage

100 Hz for frequency

0.01 μm for pulse width and treating for 15 min. The ECSP is verified to be safe.