Objective     To observe the effects of glucose on phospholipase C (PLC) activity and ［Ca

2+

］

i

in B cell line βHC9 and determine the role of PLC in glucose-stimulated insulin secretion(GSIS).      Methods     βHC9 cells were pretreated with various concentrations of glucose

then collected for assay of PLC activity and ［Ca

2+

］

i

by using［

3

H］-IP and Fura-2/AM methods respectively.      Results     When glucose concentration was 1

5

10

15

20 mmol/L

the PLC activity were 8.1、11.0、16.7、19.8、26.4 Bq/ml  (

P

<

0.05)

and the［Ca

2+

］

i

  were 82

95

128

150 and 161 nmol/L [no significant differences was seen in group 1 and 2

and group 4 and 5

while there were significant differences in the other groups (

P

<

0.05)

]

. Correlation was found between the PLC activity and the ［Ca

2+

］

i

(

P

<

0.01).      Conclusion     Glucose can change PLC activity and ［Ca

2+

］

i

in βHC9 cells

and PLC and Ca

2+

may mediate signal transduction pathway of GSIS in B cells.