Value of lymphocyte immunophenotype of liver allograft in diagnosis of acute cellular rejection

CHENG Long; YAN Xiao-chu; YANG Zhan-yu; ZHANG Rong; DUAN Guang-jie; YANG Zhi-qing; XU Sen-lin

您当前的位置：

首页 >

文章列表页 >

Value of lymphocyte immunophenotype of liver allograft in diagnosis of acute cellular rejection

更新时间：2025-01-15

- Value of lymphocyte immunophenotype of liver allograft in diagnosis of acute cellular rejection
- Journal of Army Medical University Vol. 29, Issue 6, Pages: 541-543(2007)
- 作者机构：
  
  第三军医大学西南医院：病理学研究所，全军肝胆外科研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.4;R392.12;R657.3
- Published：2007，
  
  Published Online：30 March 2007，
- 稿件说明：
移动端阅览
CHENG LONG, YAN XIAO-CHU, YANG ZHAN-YU, et al. Value of lymphocyte immunophenotype of liver allograft in diagnosis of acute cellular rejection. [J]. Journal of army medical university, 2007, 29(6): 541-543.
DOI：

CHENG LONG, YAN XIAO-CHU, YANG ZHAN-YU, et al. Value of lymphocyte immunophenotype of liver allograft in diagnosis of acute cellular rejection. [J]. Journal of army medical university, 2007, 29(6): 541-543. DOI：

摘要

目的检测移植肝组织浸润淋巴细胞的免疫表型，分析不同表型淋巴细胞数量及其分布与并发症的关系。方法应用CD8、CD4、CD3、CD20、CD45RO及CD45RA单抗对108例次移植肝活检组织进行免疫组化染色，对比形态学观察，分析不同区域浸润淋巴细胞的免疫表型。结果排斥组62例次，非排斥组46例次。排斥组汇管区CD3

、CD8

、CD20

细胞浸润程度均显著高于非排斥组（

0.01），两组间各型淋巴细胞在小叶内浸润程度均无显著差异（

>0.05）。排斥反应组中，CD8

细胞浸润程度显著高于CD4

细胞（

0.05）。排斥组CD3

、CD8

、CD45RO

细胞胆管或血管浸润率显著高于非排斥组（

0.01）。排斥组中，汇管区CD8

和CD45RO

细胞浸润程度与排斥反应组织学分级显著相关（

值分别为7.481

9.103，

0.01）。结论移植肝活检组织中淋巴细胞免疫学分型对排斥反应的诊断和鉴别诊断具有一定的指导意义。

Abstract

Objective To investigate the lymphocyte immunophenotype in liver allograft and analyze the relationship between complications and the distribution or quantity of different lymphocytes. Methods Liver allograft specimens for biopsy were immunocytochemically stained for CD8

CD4

CD3

CD20

CD45RO and CD45RA. Lymphocyte immunophenotype was analyzed in different regions based on the liver morphology. Results T

otally 108 specimens were collected

62 diagnosed as acute cellular rejection (ACR group) and 46 as other complications (non-ACR group). CD3

cells

CD8

cells and CD20

cells of ACR group were significantly more than those of non-ACR group in portal tract (

0.01)

but not significantly different in lobule (

>0.05). In ACR group

CD8

cells were significantly more than CD4

cells in both portal tract and lobule (

0.05). CD8

cells

CD3

cells and CD45RO

cells infiltrated ducts or vessels in ACR Group more easily than those in nonACR group (

0.01). The amount of CD8

cells and CD45RO

cells was related to the degree of ACR. Conclusion The lymphocyte immunophenotype detection in liver allograft specimens is helpful in pathologic diagnosis and differential diagnosis of acute cellular rejection.

关键词

肝移植淋巴细胞免疫表型免疫组织化学移植物排斥

Keywords

liver transplantationlymphocytesimmunophenotypeimmunohistochemistrygraft rejection

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Modified primary culturing of renal clear cell carcinoma

Effects of inhibiting IRE1-XBP1 pathway in Kupffer cells in rats after liver transplant rejection

Inhibiting TIM-4 function in Kupffer cells induces iTreg cell proliferation and thus immune tolerance in mice after liver transplantation

Expression and clinical significance of pyruvate dehydrogenase kinase 4 in colon cancer

Related Author

WU Dongmei

LU Debin

LI Yuqin

HUANG Yanling

HU Zhi’an

YAO Zhongxiang

ZHANG Yan

Song Lei

Related Institution

重庆医科大学附属第二医院神经内科

中国科学院大学重庆仁济医院（重庆市第五人民医院）内分泌科

重庆大学附属中心医院（重庆市急救医疗中心）神经内科

陆军军医大学（第三军医大学）基础医学院生理学教研室

重庆市綦江区人民医院神经内科

⁰