Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma

CHEN Ying; ZHONG Ling; WANG Yong; DENG Kai-xian

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Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma

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- Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma
- Journal of Army Medical University Vol. 29, Issue 6, Pages: 530-533(2007)
- 作者机构：
  
  1. 重庆医科大学附属第二医院妇产科
  2. 广东省佛山市妇幼保健院妇科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-362;R730.23;R737.31
- Published：2007，
  
  Published Online：30 March 2007，
- 稿件说明：
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CHEN YING, ZHONG LING, WANG YONG, et al. Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma. [J]. Journal of army medical university, 2007, 29(6): 530-533.
DOI：

CHEN YING, ZHONG LING, WANG YONG, et al. Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma. [J]. Journal of army medical university, 2007, 29(6): 530-533. DOI：

摘要

目的观察pshRNA-survivin在体内是否能抑制卵巢癌移植瘤体的生长。方法建立人卵巢癌裸鼠移植瘤模型，瘤内注射pshRNA-survivin，观察肿瘤生长情况。半定量RT-PCR、免疫组织化学检测移植瘤的survivin表达情况，TUNEL检测移植瘤的细胞凋亡情况。结果 pshRNA-survivin能明显抑制肿瘤组织中survivin的表达，促进肿瘤细胞的凋亡，减缓肿瘤生长速度。结论 pshRNA-survivin明显抑制了卵巢癌裸鼠移植瘤的增殖。

Abstract

Objective To examine the therapeutic effect of RNAi-mediated survivin gene silencing on the xenograft tumor of ovarian cancer

in vivo

. Methods Subcutaneous tumors in athymic mice were induced by inoculation of SKOV

. pshRNA-survivin and lipofectamine

lipofectamine

pTZU6+1 and lipofectamine

pshRNA survivin

and physical saline were injected into the subcutaneous tumors. The growth of the xenograft tumor was observed after inoculation. The expression of survivin in the xenograft tumor was observed by RT-PCR and immunohistochemistry. The cell apoptosis in tumor tissues was detected by TUNEL. Results pshRNA-survivin significantly inhibited survivin expression

accelerated the apoptosis of tumor cells and inhibited the degree and sp

eed of SKOV

cells progressing into xenograft tumor in nude mice

in vivo

. Conclusion pshRNA-survivin significantly inhibited the proliferation of xenograft tumor of ovarian carcinoma.

关键词

Survivin卵巢肿瘤细胞株SKOV3RNA干扰裸鼠

Keywords

survivinxenograft tumorSKOV3RNA interferenceathymic mice

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