Gene silencing of VEGF inhibits growth and angiogenesis of human colon cancer HCT116 implanted in nude mice

LU Wei; NIU Jin; ZHANG Chao; LIU Tao; HAO Ying-xue; LIU Wei; YU Pei-wu

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Gene silencing of VEGF inhibits growth and angiogenesis of human colon cancer HCT116 implanted in nude mice

更新时间：2025-01-15

- Gene silencing of VEGF inhibits growth and angiogenesis of human colon cancer HCT116 implanted in nude mice
- Journal of Army Medical University Vol. 29, Issue 6, Pages: 522-525(2007)
- 作者机构：
  
  第三军医大学西南医院普通外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-362;R735.35;R394-33
- Published：2007，
  
  Published Online：30 March 2007，
- 稿件说明：
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LU WEI, NIU JIN, ZHANG CHAO, et al. Gene silencing of VEGF inhibits growth and angiogenesis of human colon cancer HCT116 implanted in nude mice. [J]. Journal of army medical university, 2007, 29(6): 522-525.
DOI：

LU WEI, NIU JIN, ZHANG CHAO, et al. Gene silencing of VEGF inhibits growth and angiogenesis of human colon cancer HCT116 implanted in nude mice. [J]. Journal of army medical university, 2007, 29(6): 522-525. DOI：

摘要

目的应用RNAi技术沉默血管内皮生长因子（vascular endothelial growth factor， VEGF）基因

探讨其对人结肠癌细胞系HCT116裸鼠移植瘤生长及其血管生成的影响。方法将自行构建的以VEGF基因为靶向表达短发夹状RNA的重组质粒(pAVU6＋27-VEGF-siRNA)转染到结肠癌HCT116细胞株中（C组），以空质粒(pAVU6＋27)转染为阴性对照（B组），经G418筛选出阳性克隆细胞，未转染质粒的HCT116细胞株为空白对照（A组），建立裸小鼠皮下移植瘤模型。通过测量移植瘤的质量、体积观察移植瘤生长；免疫组化检测瘤组织中VEGF蛋白表达及组织内的微血管密度（microvessel density，MVD）改变。结果 C组肿瘤瘤块的体积（0.169±0.009）cm

和质量（0.192±0.022）g明显小于A、B组(

0.05)。A 、B组荷瘤组织中VEGF均表达强阳性，表达位于肿瘤细胞的细胞质及细胞膜上，呈棕黄色。C组荷瘤组织中VEGF呈弱阳性表达，组间差异显著(

0.05)。C组荷瘤组织中MVD明显小于两对照组，组间差异显著(

0.05)。VEGF表达与MVD呈显著正相关(

=0.810

0.01)。结论应用RNAi技术沉默VEGF基因能明显抑制人结肠癌细胞系HCT116裸小鼠移植瘤的生长，其机制与其抑制移植瘤中VEGF的表达和瘤组织内血管生成有关。

Abstract

Objective To observe the growth and angiogenesis of human colon cancer HCT116 implanted in nude mice after silencing VEGF gene expression with RNAi technique. Methods The sub-cloned recombinant plasmid shRNA (pAVU6＋27-VEGF-siRNA) and empty plasmid (pAVU6＋27) were transfected into human colon carcinoma cell l

ine HCT116 with DOTAP method

respectively named as group C and group B. The positive transfected cell clones were screened with G418. The cells receiving no plasmid transfection served as control (group A). Tumorigenicity and tumor growth of transfected cells were studied in a mouse model. VEGF expression and microvessel density (MVD) in tumor tissue were measured by immunohistochemistry. Results The mean volume and weight of the implanted tumor in group C [(0.169±0.009) cm

(0.192±0.022) g

]

were significantly lower than group A [(0.256±0.016) cm

(0.324±0.026) g

]

and group B [(0.248±0.014) cm

(0.316±0.015) g

]

(

0.05). The protein expressions of VEGF in group C were significantly decreased as compared with those in group A and group B (

0.05)

but no significant difference between group A and group B (

>0.05). Moreover

MVD in group C was also decreased as compared with that in group A and group B (

0.05)

but no significant difference between group A and group B (

>0.05). There was a positive correlation between VEGF and MVD (

=0.810

0.01). Conclusion Silencing VEGF gene can decrease VEGF gene expressions

suppress tumor angiogenesis and inhibit tumor growth in nude mice bearing human colon cancer cell line HCT116.

关键词

基因沉默血管内皮生长因子移植瘤血管生成

Keywords

Gene silencingvascular endothelial growth factorxenograft tumorangiogenesis

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