Relationship between antitumor effect of valdecoxib and COX-2 expression

LI Jun-xia; GAO Hai-xia; SU Su-wen; WANG Yong-li

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Relationship between antitumor effect of valdecoxib and COX-2 expression

更新时间：2025-01-15

- Relationship between antitumor effect of valdecoxib and COX-2 expression
- Journal of Army Medical University Vol. 29, Issue 3, Pages: 243-246(2007)
- 作者机构：
  
  河北医科大学基础医学部药理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-36;R965;R979.1
- Published：2007，
  
  Published Online：15 February 2007，
- 稿件说明：
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LI JUN-XIA, GAO HAI-XIA, SU SU-WEN, et al. Relationship between antitumor effect of valdecoxib and COX-2 expression. [J]. Journal of army medical university, 2007, 29(3): 243-246.
DOI：

LI JUN-XIA, GAO HAI-XIA, SU SU-WEN, et al. Relationship between antitumor effect of valdecoxib and COX-2 expression. [J]. Journal of army medical university, 2007, 29(3): 243-246. DOI：

摘要

目的探讨戊地昔布对肿瘤的抑制作用及其与COX-2是否有关。方法用Western blotting和免疫细胞化学检测细胞COX-2的表达。用MTT法测定药物对细胞增殖的影响。用ELISA测定PGE

（前列腺素E

）含量。结果 ①BGC-823、HGC-27和SK-OV-3细胞均无COX-2表达

clone 26 COX-2表达阳性。②戊地昔布可明显抑制4种肿瘤细胞的生长，对BGC-823、HGC-27、SK-OV-3和clone 26生长抑制的IC

分别为：110.7、99.2、113.3、117.6 μmol/L。③ 3种药物对BGC-823和clone 26细胞生长抑制程度由高到低的顺序，为戊地昔布、SC-560（选择性COX-1抑制剂）和吲哚美辛（非选择性COX-2抑制剂）。④PGE

本身对BGC-823和clone 26细胞的生长没有影响，也不拮抗戊地昔布、SC-560和吲哚美辛对细胞的生长抑制作用。⑤戊地昔布和吲哚美辛抑制细胞生长作用和抑制COX-2的作用不平行，在不影响细胞生长的浓度时就明显降低clone 26上清的PGE

含量。结论戊地昔布抑制肿瘤细胞生长的作用与COX-2无关。

Abstract

Objective To evaluate the inhibitory effect of valdecoxib on the growth of the cancer cell lines and involvement of COX-2 in this inhibition. Methods Western blotting and immunocytochemistry were used to detect the expression of COX-2. MTT assay was used to determine inhibitory effect of the drugs on the cell growth. The content of PGE

in cell medium was determined with PGE

ELISA kit. Results ①Clone 26 cells expressed high levels of COX-2

wherea

s BGC-823，HGC-27 and SK-OV-3 cell had no COX-2 expression. ②Valdecoxib inhibited the growth of BGC-823

HGC-27

SK-OV-3 and clone 26 cells

with a IC

of 110.7

99.2

113.3

117.6 μmol/L

respectively. ③The inhibitory effect of these drugs on BGC-823 and clone 26 cell was in the descending order of valdecoxib

SC-560 and indomethacin. ④PGE

did not antagonize the effect of valdecoxib

SC-560 and indomethacin on BGC-823 and clone 26 cells. ⑤The inhibitory effect of valdecoxib and indomethacin on the growth of clone 26 cells was not compatible with that on PGE

. Conclusion The inhibitory effect of valdecoxib on cell growth is not related to its effect on COX-2.

关键词

戊地昔布肿瘤COX-2SC-560吲哚美辛

Keywords

valdecoxibtumorCOX-2SC-560indomethacin

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