Effects of different concentrations of glucose on PPARδ expression in INS-1 cells

JIANG You-zhao; ZHANG Ling; ZHENG Jiang; CHEN Bing

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Effects of different concentrations of glucose on PPARδ expression in INS-1 cells

更新时间：2025-01-15

- Effects of different concentrations of glucose on PPARδ expression in INS-1 cells
- Journal of Army Medical University Vol. 29, Issue 3, Pages: 247-249(2007)
- 作者机构：
  
  第三军医大学西南医院：内分泌科，门诊部，中心实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.25;R335.6;R392.6
- Published：2007，
  
  Published Online：15 February 2007，
- 稿件说明：
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JIANG YOU-ZHAO, ZHANG LING, ZHENG JIANG, et al. Effects of different concentrations of glucose on PPARδ expression in INS-1 cells. [J]. Journal of army medical university, 2007, 29(3): 247-249.
DOI：

JIANG YOU-ZHAO, ZHANG LING, ZHENG JIANG, et al. Effects of different concentrations of glucose on PPARδ expression in INS-1 cells. [J]. Journal of army medical university, 2007, 29(3): 247-249. DOI：

摘要

目的研究不同葡萄糖浓度对大鼠INS-1 细胞PPARδ表达的影响，以初步阐明PPARδ在胰岛细胞糖脂代谢关联中的作用。方法分别用含3、11、20 mmol/L葡萄糖的RPMI1640培养基，培养INS-1细胞24 h后，冻融法裂解细胞，检测INS-1细胞内的甘油三酯水平。提取RNA和核蛋白，RT-PCR半定量检测PPARδ mRNA，Western blot 检测PPARδ蛋白表达。结果随着葡萄糖浓度的升高INS-1细胞内甘油三酯含量增高，而PPARδ mRNA和蛋白的表达均明显降低。结论葡萄糖促进，INS-1细胞内甘油三酯沉积，而PPARδ的表达下降可能是高糖条件下胰岛细胞脂质沉积的重要原因。

Abstract

Objectives To observe the effects of glucose at different concentrations on the expression of PPARδ in the rat insulinoma INS-1 cells so as to illustrate the function of PPARδ in the cross-talk of lipid metabolism with glucose metabolism in pancreatic cells. Methods After cultured in RPMI1640 media containing 3

11 and 20 mmol/L glucose respectively for 1 h

the cells were used to measure the content of intracellular triglyceride. The RNA and nuclear protein of INS-1 cells were extracted

and then the expression of PPARδ mRNA was detected by semi-quantity RT-PCR and the PPARδ protein was analyzed by Western blotting. Results With the increment of the concentrations of glucose

the content of intracellular triglyceride in the cultured cells was elevated

while the expressions of PPARδ mRNA and protein were both down-regulated. Conclusion Glucose promotes triglyceride deposition in INS-1 cells

and the down-regulation of PPARδ presumably contributes to the lipid deposition in INS-1 cells during hyperglycemic states.

关键词

PPARδINS-1细胞RT-PCRWestern blot

Keywords

PPARδINS-1 cellsRT-PCRWestern blotting

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